The role of Raf-1 in radiation resistance of human hepatocellular carcinoma Hep G2 cells

W. Y. Tang, Sophia P.Y. Chau, W. P. Tsang, S. K. Kong, T. T. Kwok

Research output: Contribution to journalArticle

Abstract

Development of radiation resistance is one of the major reasons that cancer cells do not respond to radiotherapy and the mechanism for resistance is still not clear. Two sublines of human hepatocellular carcinoma Hep G2 cells were established from cells that survived two different irradiation regimes, 2 Gy for 10 days or 10 Gy for 2 days, respectively. Using MTT assay, the radiation conditioned cells were found to be more resistant to gamma-irradiation and have a greater extent of potentially lethal damage repair (PLDR) for radiation than the parent cells. By Western blot analysis, the radiation-conditioned cells were found to overexpress Raf-1 which is known to regulate the radiation-resistance of cells. Inhibition of Raf-1 expression by antisense oligonucleotides increased the radiation sensitivity of the radiation-conditioned cells while inhibitors of Ras (L744,832), PI3K (LY294002) and p38 (SB203580) had no effect. Moreover, antisense Raf-1 oligonucleotides also decreased the radiation induced PLDR capacity of the radiation conditioned cells. It is therefore suggested that Raf-1 may induce radiation resistance through an increase in radiation induced PLDR capacity in Hep G2 cells.

Original languageEnglish (US)
Pages (from-to)1349-1354
Number of pages6
JournalOncology Reports
Volume12
Issue number6
StatePublished - Dec 1 2004

Keywords

  • Hepatocellular carcinoma
  • Radiation resistance
  • Raf-1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Tang, W. Y., Chau, S. P. Y., Tsang, W. P., Kong, S. K., & Kwok, T. T. (2004). The role of Raf-1 in radiation resistance of human hepatocellular carcinoma Hep G2 cells. Oncology Reports, 12(6), 1349-1354.