Abstract
Objectives: The PI3k/Akt pathway has been associated with the development and progression of bladder tumors, with most studies focused on papillary or muscle-invasive tumors. We sought to characterize the expression patterns of the PI3K/Akt pathway in a large cohort of high-risk preinvasive carcinoma in situ (CIS) tumors of the bladder. Our goal was to understand whether PI3K/Akt pathway alterations associated with CIS resemble early- or late-stage bladder cancers. Material and methods: We evaluated tissue specimens from 97 patients with CIS of the bladder, of which 14 had a concomitant papillary tumor. All patients were treated with intravesical bacillus Calmette-Guerin. All specimens were evaluated for PTEN, p-AKT, and p-S6 immunoreactivity. Markers were evaluated for percentage and intensity of staining and were scored using a 0 to 3+grading system. Results: PTEN staining was noted as least intense in 67% of tumor specimens and 22% of normal urothelium. P-Akt and p-S6 had intense staining in 77% and 90% of tumor specimens vs. 44% and 68% in normal tissue, respectively. Low-intensity staining for PTEN at 12 months correlated with higher recurrence risk (P = 0.026). Conclusion: We describe a large cohort of CIS bladder tumors with decreased staining intensity of PTEN and increased staining intensity of p-AKT and p-S6, similar to high-grade and high-stage papillary tumors. Low-intensity staining of PTEN at 12 months was associated with an increased risk of recurrence.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 657-662 |
| Number of pages | 6 |
| Journal | Urologic Oncology: Seminars and Original Investigations |
| Volume | 32 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2014 |
| Externally published | Yes |
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Keywords
- Bladder cancer
- Carcinoma in situ
- PTEN
ASJC Scopus subject areas
- Oncology
- Urology
- Medicine(all)
Cite this
The role of PTEN tumor suppressor pathway staining in carcinoma in situ of the bladder. / Sfakianos, John P.; Lin Gellert, Lan; Maschino, Alexandra; Gotto, Geoffrey T.; Kim, Philip H.; Al-Ahmadie, Hikmat; Bochner, Bernard H.
In: Urologic Oncology: Seminars and Original Investigations, Vol. 32, No. 5, 2014, p. 657-662.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The role of PTEN tumor suppressor pathway staining in carcinoma in situ of the bladder
AU - Sfakianos, John P.
AU - Lin Gellert, Lan
AU - Maschino, Alexandra
AU - Gotto, Geoffrey T.
AU - Kim, Philip H.
AU - Al-Ahmadie, Hikmat
AU - Bochner, Bernard H.
PY - 2014
Y1 - 2014
N2 - Objectives: The PI3k/Akt pathway has been associated with the development and progression of bladder tumors, with most studies focused on papillary or muscle-invasive tumors. We sought to characterize the expression patterns of the PI3K/Akt pathway in a large cohort of high-risk preinvasive carcinoma in situ (CIS) tumors of the bladder. Our goal was to understand whether PI3K/Akt pathway alterations associated with CIS resemble early- or late-stage bladder cancers. Material and methods: We evaluated tissue specimens from 97 patients with CIS of the bladder, of which 14 had a concomitant papillary tumor. All patients were treated with intravesical bacillus Calmette-Guerin. All specimens were evaluated for PTEN, p-AKT, and p-S6 immunoreactivity. Markers were evaluated for percentage and intensity of staining and were scored using a 0 to 3+grading system. Results: PTEN staining was noted as least intense in 67% of tumor specimens and 22% of normal urothelium. P-Akt and p-S6 had intense staining in 77% and 90% of tumor specimens vs. 44% and 68% in normal tissue, respectively. Low-intensity staining for PTEN at 12 months correlated with higher recurrence risk (P = 0.026). Conclusion: We describe a large cohort of CIS bladder tumors with decreased staining intensity of PTEN and increased staining intensity of p-AKT and p-S6, similar to high-grade and high-stage papillary tumors. Low-intensity staining of PTEN at 12 months was associated with an increased risk of recurrence.
AB - Objectives: The PI3k/Akt pathway has been associated with the development and progression of bladder tumors, with most studies focused on papillary or muscle-invasive tumors. We sought to characterize the expression patterns of the PI3K/Akt pathway in a large cohort of high-risk preinvasive carcinoma in situ (CIS) tumors of the bladder. Our goal was to understand whether PI3K/Akt pathway alterations associated with CIS resemble early- or late-stage bladder cancers. Material and methods: We evaluated tissue specimens from 97 patients with CIS of the bladder, of which 14 had a concomitant papillary tumor. All patients were treated with intravesical bacillus Calmette-Guerin. All specimens were evaluated for PTEN, p-AKT, and p-S6 immunoreactivity. Markers were evaluated for percentage and intensity of staining and were scored using a 0 to 3+grading system. Results: PTEN staining was noted as least intense in 67% of tumor specimens and 22% of normal urothelium. P-Akt and p-S6 had intense staining in 77% and 90% of tumor specimens vs. 44% and 68% in normal tissue, respectively. Low-intensity staining for PTEN at 12 months correlated with higher recurrence risk (P = 0.026). Conclusion: We describe a large cohort of CIS bladder tumors with decreased staining intensity of PTEN and increased staining intensity of p-AKT and p-S6, similar to high-grade and high-stage papillary tumors. Low-intensity staining of PTEN at 12 months was associated with an increased risk of recurrence.
KW - Bladder cancer
KW - Carcinoma in situ
KW - PTEN
UR - http://www.scopus.com/inward/record.url?scp=84902532799&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902532799&partnerID=8YFLogxK
U2 - 10.1016/j.urolonc.2014.02.003
DO - 10.1016/j.urolonc.2014.02.003
M3 - Article
C2 - 24840867
AN - SCOPUS:84902532799
VL - 32
SP - 657
EP - 662
JO - Urologic Oncology
JF - Urologic Oncology
SN - 1078-1439
IS - 5
ER -