The role of forkhead box Q1 transcription factor in ovarian epithelial carcinomas

Research output: Contribution to journalArticle

Abstract

The role of the forkhead box Q1 (FOXQ1) transcription factor in cancer pathogenesis has recently emerged. Overexpression of FOXQ1 has been found in a variety of human cancers, and its upregulation has been associated with poor prognosis in colorectal, breast, and non-small cell lung carcinomas. However, the molecular mechanism underlying how FOXQ1 contributes to ovarian epithelial carcinomas remains unclear. To this end, we analyzed gene expression levels in ovarian cancer tissues and cell lines and demonstrated a higher expression level of FOXQ1 in epithelial ovarian cancer cells than that in normal epithelial cells. We then used a human ovarian cancer cell line, SKOV3, which expressed a higher level of FOXQ1, as a cell model to investigate the biological effects of FOXQ1 by using RNA interference. Silencing of FOXQ1 expression using a shRNA knockdown approach affected the expression of several cell cycle regulators, leading to suppressed cell proliferation, reduced cell motility/invasion, and upregulation of epithelial cell markers and the downregulation of mesenchymal cell markers. Taken together, these results suggest that FOXQ1 expression is essential to maintain cell proliferation, motility/invasion, and epithelial-mesenchymal transition phenotypes in ovarian cancer cells.

Original languageEnglish (US)
Pages (from-to)13881-13893
Number of pages13
JournalInternational Journal of Molecular Sciences
Volume13
Issue number11
DOIs
StatePublished - 2012

Fingerprint

Forkhead Transcription Factors
Transcription factors
boxes
Transcription Factors
cancer
Cells
Ovarian Neoplasms
Carcinoma
Cell Movement
Up-Regulation
Epithelial Cells
Cell Proliferation
Cell proliferation
Cell Line
Epithelial-Mesenchymal Transition
locomotion
RNA Interference
cultured cells
Non-Small Cell Lung Carcinoma
markers

Keywords

  • FOXQ1
  • Invasion
  • Migration
  • Ovarian cancer
  • Survival

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

Cite this

The role of forkhead box Q1 transcription factor in ovarian epithelial carcinomas. / Gao, Min; Shih, Ie Ming; Wang, Tian-Li.

In: International Journal of Molecular Sciences, Vol. 13, No. 11, 2012, p. 13881-13893.

Research output: Contribution to journalArticle

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