The role of eutral lipid nanospheres in Plasmodium falciparum haem crystallization

John M. Pisciotta, Isabelle Coppens, Abhai Tripathi, Peter F. Scholl, Joel Shuman, Sunil Bajad, Vladimir Shulaev, David J Sullivan

Research output: Contribution to journalArticle

Abstract

The intraerythrocytic malaria parasite constructs an intracellular haem crystal, called haemozoin, within an acidic digestive vacuole where haemoglobin is degraded. Haem crystallization is the target of the widely used antimalarial quinoline drugs. The intracellular mechanism of molecular initiation of haem crystallization, whether by proteins, polar membrane lipids or by neutral lipids, has not been fully substantiated. In the present study, we show neutral lipid predominant nanospheres, which envelop haemozoin inside Plasmodium falciparum digestive vacuoles. Subcellular fractionation of parasite-derived haemozoin through a dense 1.7 M sucrose cushion identifies monoacylglycerol and diacylglycerol neutral lipids as well as some polar lipids in close association with the purified haemozoin. Global MS lipidomics detects monopalmitic glycerol and monostearic glycerol, but not mono-oleic glycerol, closely associated with haemozoin. The complex neutral lipid mixture rapidly initiates haem crystallization, with reversible pH-dependent quinoline inhibition associated with quinoline entry into the neutral lipid microenvironment. Neutral lipid nanospheres both enable haem crystallization in the presence of high globin concentrations and protect haem from H2O2 degradation. Conceptually, the present study shifts the intracellular microenvironment of haem crystallization and quinoline inhibition from a polar aqueous location to a non-polar neutral lipid nanosphere able to exclude water for efficient haem crystallization.

Original languageEnglish (US)
Pages (from-to)197-204
Number of pages8
JournalBiochemical Journal
Volume402
Issue number1
DOIs
StatePublished - Feb 15 2007

Fingerprint

Nanospheres
Plasmodium falciparum
Crystallization
Heme
Lipids
Glycerol
Vacuoles
Parasites
Monoglycerides
Globins
Diglycerides
Antimalarials
Membrane Lipids
Fractionation
Malaria
Sucrose
Hemoglobins
hemozoin
Association reactions
Degradation

Keywords

  • Haem crystallization
  • Haemozoin
  • Lipidomics
  • Malaria
  • Neutral lipid
  • Plasmodium falciparum
  • Quinoline

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

The role of eutral lipid nanospheres in Plasmodium falciparum haem crystallization. / Pisciotta, John M.; Coppens, Isabelle; Tripathi, Abhai; Scholl, Peter F.; Shuman, Joel; Bajad, Sunil; Shulaev, Vladimir; Sullivan, David J.

In: Biochemical Journal, Vol. 402, No. 1, 15.02.2007, p. 197-204.

Research output: Contribution to journalArticle

Pisciotta, John M. ; Coppens, Isabelle ; Tripathi, Abhai ; Scholl, Peter F. ; Shuman, Joel ; Bajad, Sunil ; Shulaev, Vladimir ; Sullivan, David J. / The role of eutral lipid nanospheres in Plasmodium falciparum haem crystallization. In: Biochemical Journal. 2007 ; Vol. 402, No. 1. pp. 197-204.
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