TY - JOUR
T1 - The role of E-cadherin in the motility and invasion of implantation site intermediate trophoblast
AU - Shih, Ie Ming
AU - Hsu, Mei Yu
AU - Oldt, Robert J.
AU - Herlyn, Meenhard
AU - Gearhart, John D.
AU - Kurman, Robert J.
N1 - Funding Information:
This investigation was supported by the Richard W. TeLinde Research Award (M020-440-0107) from the Division of Gynecologic Pathology, Department of Pathology, The Johns Hopkins Hospital and NIH grant CA76674 (M. Herlyn).
PY - 2002/11
Y1 - 2002/11
N2 - During early pregnancy, intermediate (extravillous) trophoblast infiltrates the basal plate and invades the spiral arteries, a physiological process required to establish the maternal - fetal circulation. Immunostaining studies have shown that differentiation of trophoblast into this invasive subpopulation is associated with down-regulation of E-cadherin expression. To study the function of E-cadherin in trophoblast in vitro, we restored E-cadherin expression in an E-cadherin negative human implantation site intermediate trophoblastic cell line, IST-1, using a recombinant adenovirus, E-cad/Ad5 which constitutively expresses E-cadherin. In contrast to the control IST-1 cells which were individual and pleomorphic in shape, E-cad/Ad5 transduced cells were cohesive, uniform, and round. The motility and invasiveness of E-cad/Ad5 transduced IST-1 cells, as compared with the control cells, was significantly reduced. These effects were contact-dependent and were attenuated by a function-perturbing anti-E-cadherin antibody. In conclusion, our results indicate that expression of E-cadherin in IST-1 cells results in a contact-mediated inhibition of motility and invasion and suggest an important role for E-cadherin down-regulation in the intermediate trophoblast during implantation.
AB - During early pregnancy, intermediate (extravillous) trophoblast infiltrates the basal plate and invades the spiral arteries, a physiological process required to establish the maternal - fetal circulation. Immunostaining studies have shown that differentiation of trophoblast into this invasive subpopulation is associated with down-regulation of E-cadherin expression. To study the function of E-cadherin in trophoblast in vitro, we restored E-cadherin expression in an E-cadherin negative human implantation site intermediate trophoblastic cell line, IST-1, using a recombinant adenovirus, E-cad/Ad5 which constitutively expresses E-cadherin. In contrast to the control IST-1 cells which were individual and pleomorphic in shape, E-cad/Ad5 transduced cells were cohesive, uniform, and round. The motility and invasiveness of E-cad/Ad5 transduced IST-1 cells, as compared with the control cells, was significantly reduced. These effects were contact-dependent and were attenuated by a function-perturbing anti-E-cadherin antibody. In conclusion, our results indicate that expression of E-cadherin in IST-1 cells results in a contact-mediated inhibition of motility and invasion and suggest an important role for E-cadherin down-regulation in the intermediate trophoblast during implantation.
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U2 - 10.1016/S0143-4004(02)90864-7
DO - 10.1016/S0143-4004(02)90864-7
M3 - Article
C2 - 12398810
AN - SCOPUS:0036834572
SN - 0143-4004
VL - 23
SP - 706
EP - 715
JO - Placenta
JF - Placenta
IS - 10
ER -