The role of anion exchange in the uptake of Pb by human erythrocytes and Madin-Darby canine kidney cells

Desmond I. Bannon; Luisa Olivi; Joseph Bressler

doi:10.1016/S0300-483X(00)00187-6

The role of anion exchange in the uptake of Pb by human erythrocytes and Madin-Darby canine kidney cells

Desmond I. Bannon, Luisa Olivi, Joseph Bressler

Kennedy Krieger Institute

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Anion exchange (AE) plays a critical role in regulating intracellular pH in erythrocytes and epithelial cells and has been suggested to facilitate the transport of lead (Pb) across the erythrocyte cell membrane. In this study we examined the role of AE in the uptake of Pb by human erythrocytes and by Madin-Darby canine kidney (MDCK) cells, the kidney epithelial cell line. Functional AE in MDCK cells was evidenced by: increased uptake of SO₄^2- at pH 6.0 over pH 7.0, and inhibition of SO₄^2- uptake by the AE inhibitor 4, 4'-diisothiocyanostilbene-2, 2'- disulfonic acid (DIDS) as well as by non-halide anions. Accumulation of Pb into MDCK cells was time and temperature dependent. DIDS inhibited uptake of Pb into human erythrocytes but not MDCK cells. In conclusion, uptake of Pb into erythrocytes but not kidney epithelial cells occurs through AE. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English (US)
Pages (from-to)	101-107
Number of pages	7
Journal	Toxicology
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/S0300-483X(00)00187-6
State	Published - Jun 8 2000

Keywords

Anion exchange
Erythrocytes
Kidney
Lead

ASJC Scopus subject areas

Toxicology

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10.1016/S0300-483X(00)00187-6

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title = "The role of anion exchange in the uptake of Pb by human erythrocytes and Madin-Darby canine kidney cells",

abstract = "Anion exchange (AE) plays a critical role in regulating intracellular pH in erythrocytes and epithelial cells and has been suggested to facilitate the transport of lead (Pb) across the erythrocyte cell membrane. In this study we examined the role of AE in the uptake of Pb by human erythrocytes and by Madin-Darby canine kidney (MDCK) cells, the kidney epithelial cell line. Functional AE in MDCK cells was evidenced by: increased uptake of SO42- at pH 6.0 over pH 7.0, and inhibition of SO42- uptake by the AE inhibitor 4, 4'-diisothiocyanostilbene-2, 2'- disulfonic acid (DIDS) as well as by non-halide anions. Accumulation of Pb into MDCK cells was time and temperature dependent. DIDS inhibited uptake of Pb into human erythrocytes but not MDCK cells. In conclusion, uptake of Pb into erythrocytes but not kidney epithelial cells occurs through AE. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Anion exchange, Erythrocytes, Kidney, Lead",

author = "Bannon, {Desmond I.} and Luisa Olivi and Joseph Bressler",

note = "Funding Information: This work was supported by NIH grants RO1 ES08785 (J.B.), RO1 ES07980 (J.B.), and NIEHS Center Grant #03819. The authors would like to thank Angela T. Williams for assistance in the preparation of this article.",

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AU - Bressler, Joseph

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PY - 2000/6/8

Y1 - 2000/6/8

N2 - Anion exchange (AE) plays a critical role in regulating intracellular pH in erythrocytes and epithelial cells and has been suggested to facilitate the transport of lead (Pb) across the erythrocyte cell membrane. In this study we examined the role of AE in the uptake of Pb by human erythrocytes and by Madin-Darby canine kidney (MDCK) cells, the kidney epithelial cell line. Functional AE in MDCK cells was evidenced by: increased uptake of SO42- at pH 6.0 over pH 7.0, and inhibition of SO42- uptake by the AE inhibitor 4, 4'-diisothiocyanostilbene-2, 2'- disulfonic acid (DIDS) as well as by non-halide anions. Accumulation of Pb into MDCK cells was time and temperature dependent. DIDS inhibited uptake of Pb into human erythrocytes but not MDCK cells. In conclusion, uptake of Pb into erythrocytes but not kidney epithelial cells occurs through AE. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - Anion exchange (AE) plays a critical role in regulating intracellular pH in erythrocytes and epithelial cells and has been suggested to facilitate the transport of lead (Pb) across the erythrocyte cell membrane. In this study we examined the role of AE in the uptake of Pb by human erythrocytes and by Madin-Darby canine kidney (MDCK) cells, the kidney epithelial cell line. Functional AE in MDCK cells was evidenced by: increased uptake of SO42- at pH 6.0 over pH 7.0, and inhibition of SO42- uptake by the AE inhibitor 4, 4'-diisothiocyanostilbene-2, 2'- disulfonic acid (DIDS) as well as by non-halide anions. Accumulation of Pb into MDCK cells was time and temperature dependent. DIDS inhibited uptake of Pb into human erythrocytes but not MDCK cells. In conclusion, uptake of Pb into erythrocytes but not kidney epithelial cells occurs through AE. Copyright (C) 2000 Elsevier Science Ireland Ltd.

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The role of anion exchange in the uptake of Pb by human erythrocytes and Madin-Darby canine kidney cells

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