The RNA component of human telomerase

Junli Feng; Walter D. Funk; Sy Shi Wang; Scott L. Weinrich; Ariel A. Avilion; Choy Pik Chiu; Robert R. Adams; Edwin Chang; Richard C. Allsopp; Jinghua Yu; Siyuan Le; Michael D. West; Calvin B. Harley; William H. Andrews; Carol W. Greider; Bryant Villeponteau

doi:10.1126/science.7544491

The RNA component of human telomerase

Junli Feng, Walter D. Funk, Sy Shi Wang, Scott L. Weinrich, Ariel A. Avilion, Choy Pik Chiu, Robert R. Adams, Edwin Chang, Richard C. Allsopp, Jinghua Yu, Siyuan Le, Michael D. West, Calvin B. Harley, William H. Andrews, Carol W. Greider, Bryant Villeponteau

Research output: Contribution to journal › Article › peer-review

2007 Scopus citations

Abstract

Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA) - protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5′-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG)_n. Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.

Original language	English (US)
Pages (from-to)	1236-1241
Number of pages	6
Journal	Science
Volume	269
Issue number	5228
DOIs	https://doi.org/10.1126/science.7544491
State	Published - 1995
Externally published	Yes

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@article{71ffdae757ed49429f076f9e6abb891e,

title = "The RNA component of human telomerase",

abstract = "Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA) - protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5′-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG)n. Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.",

author = "Junli Feng and Funk, {Walter D.} and Wang, {Sy Shi} and Weinrich, {Scott L.} and Avilion, {Ariel A.} and Chiu, {Choy Pik} and Adams, {Robert R.} and Edwin Chang and Allsopp, {Richard C.} and Jinghua Yu and Siyuan Le and West, {Michael D.} and Harley, {Calvin B.} and Andrews, {William H.} and Greider, {Carol W.} and Bryant Villeponteau",

year = "1995",

doi = "10.1126/science.7544491",

language = "English (US)",

volume = "269",

pages = "1236--1241",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5228",

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TY - JOUR

T1 - The RNA component of human telomerase

AU - Feng, Junli

AU - Funk, Walter D.

AU - Wang, Sy Shi

AU - Weinrich, Scott L.

AU - Avilion, Ariel A.

AU - Chiu, Choy Pik

AU - Adams, Robert R.

AU - Chang, Edwin

AU - Allsopp, Richard C.

AU - Yu, Jinghua

AU - Le, Siyuan

AU - West, Michael D.

AU - Harley, Calvin B.

AU - Andrews, William H.

AU - Greider, Carol W.

AU - Villeponteau, Bryant

PY - 1995

Y1 - 1995

N2 - Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA) - protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5′-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG)n. Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.

AB - Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA) - protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5′-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG)n. Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.

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DO - 10.1126/science.7544491

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SN - 0036-8075

VL - 269

SP - 1236

EP - 1241

JO - Science

JF - Science

IS - 5228

ER -

The RNA component of human telomerase

Abstract

ASJC Scopus subject areas

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