The RNA component of human telomerase

Junli Feng, Walter D. Funk, Sy Shi Wang, Scott L. Weinrich, Ariel A. Avilion, Choy Pik Chiu, Robert R. Adams, Edwin Chang, Richard C. Allsopp, Jinghua Yu, Siyuan Le, Michael D. West, Calvin B. Harley, William H. Andrews, Carol W. Greider, Bryant Villeponteau

Research output: Contribution to journalArticle

Abstract

Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA) - protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5′-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG)n. Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.

Original languageEnglish (US)
Pages (from-to)1236-1241
Number of pages6
JournalScience
Volume269
Issue number5228
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • General

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    Feng, J., Funk, W. D., Wang, S. S., Weinrich, S. L., Avilion, A. A., Chiu, C. P., Adams, R. R., Chang, E., Allsopp, R. C., Yu, J., Le, S., West, M. D., Harley, C. B., Andrews, W. H., Greider, C. W., & Villeponteau, B. (1995). The RNA component of human telomerase. Science, 269(5228), 1236-1241. https://doi.org/10.1126/science.7544491