The restricted nature of HIV-1 tropism for cultured neural cells

Norman Sharpless, Don Gilbert, Bernard Vandercam, Jia Min Zhou, Eric Verdin, Gabriele Ronnett, Emily Friedman, Monique Dubois-Dalcq

Research output: Contribution to journalArticle

Abstract

Infection of the central nervous system by HIV-1, the agent of AIDS, is characterized by the presence of infected and giant microglial cells as well as astrocytosis, demyelination, and neuronal loss. To determine whether cells of neuroectoderm origin can be infected by HIV-1, we have inoculated primary cultures derived from adult human brain with a lymphotropic virus (LAV) or a neurotropic virus (Jr-FL) isolated from a patient with AIDS dementia. While Jr-FL invariably causes productive infection of cultured brain microglia, neither astrocytes nor oligodendrocytes became productively infected by these viral strains. Moreover, the cultured oligodendrocytes develop a normal network of processes and express differentiation antigens in the presence of an ongoing lytic infection of microglial cells. No HIV-1 proviral DNA was detected in primary astrocyte cultures devoid of microglia after inoculation of either HIV-1 strain. Similarly, the neuronal cell line HCN-1 in its differentiated state did not allow the virus to go through cycles of reverse transcription and replication. LAV, however, was able to replicate in undifferentiated HCN-1 cells. Thus, tropism of HIV-1 appears tightly restricted to only one type of differentiated cell in the CNS, the microglia.

Original languageEnglish (US)
Pages (from-to)813-825
Number of pages13
JournalVirology
Volume191
Issue number2
DOIs
StatePublished - 1992

Fingerprint

Tropism
HIV-1
Cultured Cells
Microglia
Oligodendroglia
Viruses
Astrocytes
Acquired Immunodeficiency Syndrome
Neural Plate
Central Nervous System Infections
Gliosis
Differentiation Antigens
Brain
Demyelinating Diseases
Giant Cells
Infection
Reverse Transcription
Dementia
Cell Line
DNA

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Sharpless, N., Gilbert, D., Vandercam, B., Zhou, J. M., Verdin, E., Ronnett, G., ... Dubois-Dalcq, M. (1992). The restricted nature of HIV-1 tropism for cultured neural cells. Virology, 191(2), 813-825. https://doi.org/10.1016/0042-6822(92)90257-P

The restricted nature of HIV-1 tropism for cultured neural cells. / Sharpless, Norman; Gilbert, Don; Vandercam, Bernard; Zhou, Jia Min; Verdin, Eric; Ronnett, Gabriele; Friedman, Emily; Dubois-Dalcq, Monique.

In: Virology, Vol. 191, No. 2, 1992, p. 813-825.

Research output: Contribution to journalArticle

Sharpless, N, Gilbert, D, Vandercam, B, Zhou, JM, Verdin, E, Ronnett, G, Friedman, E & Dubois-Dalcq, M 1992, 'The restricted nature of HIV-1 tropism for cultured neural cells', Virology, vol. 191, no. 2, pp. 813-825. https://doi.org/10.1016/0042-6822(92)90257-P
Sharpless N, Gilbert D, Vandercam B, Zhou JM, Verdin E, Ronnett G et al. The restricted nature of HIV-1 tropism for cultured neural cells. Virology. 1992;191(2):813-825. https://doi.org/10.1016/0042-6822(92)90257-P
Sharpless, Norman ; Gilbert, Don ; Vandercam, Bernard ; Zhou, Jia Min ; Verdin, Eric ; Ronnett, Gabriele ; Friedman, Emily ; Dubois-Dalcq, Monique. / The restricted nature of HIV-1 tropism for cultured neural cells. In: Virology. 1992 ; Vol. 191, No. 2. pp. 813-825.
@article{7c707254ee73400789b3470c52b4a04b,
title = "The restricted nature of HIV-1 tropism for cultured neural cells",
abstract = "Infection of the central nervous system by HIV-1, the agent of AIDS, is characterized by the presence of infected and giant microglial cells as well as astrocytosis, demyelination, and neuronal loss. To determine whether cells of neuroectoderm origin can be infected by HIV-1, we have inoculated primary cultures derived from adult human brain with a lymphotropic virus (LAV) or a neurotropic virus (Jr-FL) isolated from a patient with AIDS dementia. While Jr-FL invariably causes productive infection of cultured brain microglia, neither astrocytes nor oligodendrocytes became productively infected by these viral strains. Moreover, the cultured oligodendrocytes develop a normal network of processes and express differentiation antigens in the presence of an ongoing lytic infection of microglial cells. No HIV-1 proviral DNA was detected in primary astrocyte cultures devoid of microglia after inoculation of either HIV-1 strain. Similarly, the neuronal cell line HCN-1 in its differentiated state did not allow the virus to go through cycles of reverse transcription and replication. LAV, however, was able to replicate in undifferentiated HCN-1 cells. Thus, tropism of HIV-1 appears tightly restricted to only one type of differentiated cell in the CNS, the microglia.",
author = "Norman Sharpless and Don Gilbert and Bernard Vandercam and Zhou, {Jia Min} and Eric Verdin and Gabriele Ronnett and Emily Friedman and Monique Dubois-Dalcq",
year = "1992",
doi = "10.1016/0042-6822(92)90257-P",
language = "English (US)",
volume = "191",
pages = "813--825",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The restricted nature of HIV-1 tropism for cultured neural cells

AU - Sharpless, Norman

AU - Gilbert, Don

AU - Vandercam, Bernard

AU - Zhou, Jia Min

AU - Verdin, Eric

AU - Ronnett, Gabriele

AU - Friedman, Emily

AU - Dubois-Dalcq, Monique

PY - 1992

Y1 - 1992

N2 - Infection of the central nervous system by HIV-1, the agent of AIDS, is characterized by the presence of infected and giant microglial cells as well as astrocytosis, demyelination, and neuronal loss. To determine whether cells of neuroectoderm origin can be infected by HIV-1, we have inoculated primary cultures derived from adult human brain with a lymphotropic virus (LAV) or a neurotropic virus (Jr-FL) isolated from a patient with AIDS dementia. While Jr-FL invariably causes productive infection of cultured brain microglia, neither astrocytes nor oligodendrocytes became productively infected by these viral strains. Moreover, the cultured oligodendrocytes develop a normal network of processes and express differentiation antigens in the presence of an ongoing lytic infection of microglial cells. No HIV-1 proviral DNA was detected in primary astrocyte cultures devoid of microglia after inoculation of either HIV-1 strain. Similarly, the neuronal cell line HCN-1 in its differentiated state did not allow the virus to go through cycles of reverse transcription and replication. LAV, however, was able to replicate in undifferentiated HCN-1 cells. Thus, tropism of HIV-1 appears tightly restricted to only one type of differentiated cell in the CNS, the microglia.

AB - Infection of the central nervous system by HIV-1, the agent of AIDS, is characterized by the presence of infected and giant microglial cells as well as astrocytosis, demyelination, and neuronal loss. To determine whether cells of neuroectoderm origin can be infected by HIV-1, we have inoculated primary cultures derived from adult human brain with a lymphotropic virus (LAV) or a neurotropic virus (Jr-FL) isolated from a patient with AIDS dementia. While Jr-FL invariably causes productive infection of cultured brain microglia, neither astrocytes nor oligodendrocytes became productively infected by these viral strains. Moreover, the cultured oligodendrocytes develop a normal network of processes and express differentiation antigens in the presence of an ongoing lytic infection of microglial cells. No HIV-1 proviral DNA was detected in primary astrocyte cultures devoid of microglia after inoculation of either HIV-1 strain. Similarly, the neuronal cell line HCN-1 in its differentiated state did not allow the virus to go through cycles of reverse transcription and replication. LAV, however, was able to replicate in undifferentiated HCN-1 cells. Thus, tropism of HIV-1 appears tightly restricted to only one type of differentiated cell in the CNS, the microglia.

UR - http://www.scopus.com/inward/record.url?scp=0027074714&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027074714&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(92)90257-P

DO - 10.1016/0042-6822(92)90257-P

M3 - Article

C2 - 1448925

AN - SCOPUS:0027074714

VL - 191

SP - 813

EP - 825

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -