The response of osteoblast-like cells to dexamethasone and cyclic loading

Lynne C. Jones; Brian Yeoumans; David S. Hungerford; Carmelita G. Frondoza

The response of osteoblast-like cells to dexamethasone and cyclic loading

Lynne C. Jones, Brian Yeoumans, David S. Hungerford, Carmelita G. Frondoza

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The aim of this study was to investigate whether cyclic loading can alter the response of osteoblasts to dexamethasone. Proliferation of osteoblast-like cells (MG-63) was assessed by determining uptake of ³H-thymidine. Three doses of dexamethasone were tested: 0.00InM, .OOlnM, or O.lnM. Exposure to increasing levels of dexamethasone resulted in decreased proliferation of osteoblast-like cells. Although there was a slight decrease in proliferation seen in the controls (no dexamethasone) with cyclic loading, cyclic loading did not affect the response of osteoblast-like cells to dexamethasone under these test conditions. Varying loading conditions and the potential effect on metabolic and synthetic activity of the osteoblast-like cells need further study.

Original language	English (US)
Title of host publication	Technical Papers of ISA
Pages	273-277
Number of pages	5
Volume	464
State	Published - 2006
Event	43rd Annual Rocky Mountain Bioengineering Symposium and 43rd International ISA Biomedical Sciences Instrumentation Symposium - Terre Haute, IN, United States Duration: Apr 7 2006 → Apr 9 2006

Other

Other	43rd Annual Rocky Mountain Bioengineering Symposium and 43rd International ISA Biomedical Sciences Instrumentation Symposium
Country/Territory	United States
City	Terre Haute, IN
Period	4/7/06 → 4/9/06

Keywords

Cellular response
Cyclic tensile strain
Osteoblasts
Osteonecrosis
Osteoporosis

ASJC Scopus subject areas

Control and Systems Engineering
Electrical and Electronic Engineering
Computer Science Applications

Cite this

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title = "The response of osteoblast-like cells to dexamethasone and cyclic loading",

abstract = "The aim of this study was to investigate whether cyclic loading can alter the response of osteoblasts to dexamethasone. Proliferation of osteoblast-like cells (MG-63) was assessed by determining uptake of 3H-thymidine. Three doses of dexamethasone were tested: 0.00InM, .OOlnM, or O.lnM. Exposure to increasing levels of dexamethasone resulted in decreased proliferation of osteoblast-like cells. Although there was a slight decrease in proliferation seen in the controls (no dexamethasone) with cyclic loading, cyclic loading did not affect the response of osteoblast-like cells to dexamethasone under these test conditions. Varying loading conditions and the potential effect on metabolic and synthetic activity of the osteoblast-like cells need further study.",

keywords = "Cellular response, Cyclic tensile strain, Osteoblasts, Osteonecrosis, Osteoporosis",

author = "Jones, {Lynne C.} and Brian Yeoumans and Hungerford, {David S.} and Frondoza, {Carmelita G.}",

year = "2006",

language = "English (US)",

volume = "464",

pages = "273--277",

booktitle = "Technical Papers of ISA",

note = "43rd Annual Rocky Mountain Bioengineering Symposium and 43rd International ISA Biomedical Sciences Instrumentation Symposium ; Conference date: 07-04-2006 Through 09-04-2006",

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TY - GEN

T1 - The response of osteoblast-like cells to dexamethasone and cyclic loading

AU - Jones, Lynne C.

AU - Yeoumans, Brian

AU - Hungerford, David S.

AU - Frondoza, Carmelita G.

PY - 2006

Y1 - 2006

N2 - The aim of this study was to investigate whether cyclic loading can alter the response of osteoblasts to dexamethasone. Proliferation of osteoblast-like cells (MG-63) was assessed by determining uptake of 3H-thymidine. Three doses of dexamethasone were tested: 0.00InM, .OOlnM, or O.lnM. Exposure to increasing levels of dexamethasone resulted in decreased proliferation of osteoblast-like cells. Although there was a slight decrease in proliferation seen in the controls (no dexamethasone) with cyclic loading, cyclic loading did not affect the response of osteoblast-like cells to dexamethasone under these test conditions. Varying loading conditions and the potential effect on metabolic and synthetic activity of the osteoblast-like cells need further study.

AB - The aim of this study was to investigate whether cyclic loading can alter the response of osteoblasts to dexamethasone. Proliferation of osteoblast-like cells (MG-63) was assessed by determining uptake of 3H-thymidine. Three doses of dexamethasone were tested: 0.00InM, .OOlnM, or O.lnM. Exposure to increasing levels of dexamethasone resulted in decreased proliferation of osteoblast-like cells. Although there was a slight decrease in proliferation seen in the controls (no dexamethasone) with cyclic loading, cyclic loading did not affect the response of osteoblast-like cells to dexamethasone under these test conditions. Varying loading conditions and the potential effect on metabolic and synthetic activity of the osteoblast-like cells need further study.

KW - Cellular response

KW - Cyclic tensile strain

KW - Osteoblasts

KW - Osteonecrosis

KW - Osteoporosis

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M3 - Conference contribution

VL - 464

SP - 273

EP - 277

BT - Technical Papers of ISA

T2 - 43rd Annual Rocky Mountain Bioengineering Symposium and 43rd International ISA Biomedical Sciences Instrumentation Symposium

Y2 - 7 April 2006 through 9 April 2006

ER -

The response of osteoblast-like cells to dexamethasone and cyclic loading

Abstract

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Keywords

ASJC Scopus subject areas

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