The resolution of platelet serologic problems using Western blotting

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Abstract

Serologic identification of anti‐PLAI and other platelet specific antibodies is difficult because other platelet reactive antibodies (e.g. anti‐HLA) are commonly found in the samples. We have developed and evaluated a method using Western blotting to identify platelet specific antibodies. Since anti‐PLAI is the most frequently encountered antibody, we used this antibody to validate the method. The PLAI antigen was found on a 95,000 Dalton protein, confirmed as glycoprotein IIIa by two‐dimensional electrophoresis. The usefulness of Western blotting in resolving difficult platelet serologic problems was compared to a radiolabeled antiglobulin test using intact platelets. Our results showed that all sera with anti‐PLAI identified by serology were confirmed by Western blotting. In addition, one anti‐PLAI was detectable only by the proposed method. Western blotting was also as useful as a serologic method to PLAI type platelets. HLA antibodies were not demonstrable by this technique. In summary, Western blotting is a highly specific and sensitive way of identifying anti‐PLAI in a variety of clinical situations. This approach may be used to identify antibodies to other platelet specific antigens and to standardize platelet antigen typing sera.

Original languageEnglish (US)
Pages (from-to)257-268
Number of pages12
JournalTissue Antigens
Volume28
Issue number5
DOIs
StatePublished - Nov 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

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