The renal cGMP-gated cation channel: Its molecular structure and physiological role

David E. McCoy, Sandra E. Guggino, Bruce A. Stanton

Research output: Contribution to journalArticle

Abstract

Cyclic nucleotide-gated cation channels, which are permeable to monovalent and divalent cations, are expressed in a number of tissues. cDNAs encoding cGMP-gated cation channel subunits have been cloned in retinal rods, cones, olfactory neuroepithelium, pineal gland, aorta, testis, heart, and most recently kidney. Patch clamp studies have identified and characterized cGMP-gated cation channels in the cortical collecting duct (CCD) and inner medullary collecting duct (IMCD). cGMP-gated cation channels in kidney share many biophysical and molecular properties with the retinal rod cGMP-gated channel. However, unlike the retinal rod channel, the cGMP-gated cation channel in kidney is inhibited by cGMP and stimulated by increased calcium levels. In the IMCD the cGMP-gated cation channel mediates electrogenic sodium absorption which is inhibited by ANP via cGMP. Recently, cGMP-gated cation channel poly(A+) RNA has been identified in other nephron segments by RT-PCR and in situ PCR hybridization. Furthermore, cGMP-gated cation channel protein has also been identified in all nephron segments by Western blot analysis. These observations suggest that cGMP-gated cation channels, or closely related gene products, may play an important physiological role in all nephron segments. Hormones that increase intracellular cGMP may regulate sodium, and perhaps calcium, uptake in nephron segments proximal to the IMCD. Increases in cell sodium and calcium may regulate other transport and signaling pathways.

Original languageEnglish (US)
Pages (from-to)1125-1133
Number of pages9
JournalKidney International
Volume48
Issue number4
StatePublished - Oct 1995
Externally publishedYes

Fingerprint

Molecular Structure
Cations
Kidney
Nephrons
Retinal Rod Photoreceptor Cells
Sodium
Calcium
Cyclic Nucleotide-Gated Cation Channels
Retinal Cone Photoreceptor Cells
Monovalent Cations
Polymerase Chain Reaction
Vertebrate Photoreceptor Cells
Pineal Gland
Divalent Cations
Atrial Natriuretic Factor
In Situ Hybridization
Aorta
Testis
Complementary DNA
Western Blotting

ASJC Scopus subject areas

  • Nephrology

Cite this

McCoy, D. E., Guggino, S. E., & Stanton, B. A. (1995). The renal cGMP-gated cation channel: Its molecular structure and physiological role. Kidney International, 48(4), 1125-1133.

The renal cGMP-gated cation channel : Its molecular structure and physiological role. / McCoy, David E.; Guggino, Sandra E.; Stanton, Bruce A.

In: Kidney International, Vol. 48, No. 4, 10.1995, p. 1125-1133.

Research output: Contribution to journalArticle

McCoy, DE, Guggino, SE & Stanton, BA 1995, 'The renal cGMP-gated cation channel: Its molecular structure and physiological role', Kidney International, vol. 48, no. 4, pp. 1125-1133.
McCoy, David E. ; Guggino, Sandra E. ; Stanton, Bruce A. / The renal cGMP-gated cation channel : Its molecular structure and physiological role. In: Kidney International. 1995 ; Vol. 48, No. 4. pp. 1125-1133.
@article{6ee6e98507ba4ce6a30128d2be92026a,
title = "The renal cGMP-gated cation channel: Its molecular structure and physiological role",
abstract = "Cyclic nucleotide-gated cation channels, which are permeable to monovalent and divalent cations, are expressed in a number of tissues. cDNAs encoding cGMP-gated cation channel subunits have been cloned in retinal rods, cones, olfactory neuroepithelium, pineal gland, aorta, testis, heart, and most recently kidney. Patch clamp studies have identified and characterized cGMP-gated cation channels in the cortical collecting duct (CCD) and inner medullary collecting duct (IMCD). cGMP-gated cation channels in kidney share many biophysical and molecular properties with the retinal rod cGMP-gated channel. However, unlike the retinal rod channel, the cGMP-gated cation channel in kidney is inhibited by cGMP and stimulated by increased calcium levels. In the IMCD the cGMP-gated cation channel mediates electrogenic sodium absorption which is inhibited by ANP via cGMP. Recently, cGMP-gated cation channel poly(A+) RNA has been identified in other nephron segments by RT-PCR and in situ PCR hybridization. Furthermore, cGMP-gated cation channel protein has also been identified in all nephron segments by Western blot analysis. These observations suggest that cGMP-gated cation channels, or closely related gene products, may play an important physiological role in all nephron segments. Hormones that increase intracellular cGMP may regulate sodium, and perhaps calcium, uptake in nephron segments proximal to the IMCD. Increases in cell sodium and calcium may regulate other transport and signaling pathways.",
author = "McCoy, {David E.} and Guggino, {Sandra E.} and Stanton, {Bruce A.}",
year = "1995",
month = "10",
language = "English (US)",
volume = "48",
pages = "1125--1133",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - The renal cGMP-gated cation channel

T2 - Its molecular structure and physiological role

AU - McCoy, David E.

AU - Guggino, Sandra E.

AU - Stanton, Bruce A.

PY - 1995/10

Y1 - 1995/10

N2 - Cyclic nucleotide-gated cation channels, which are permeable to monovalent and divalent cations, are expressed in a number of tissues. cDNAs encoding cGMP-gated cation channel subunits have been cloned in retinal rods, cones, olfactory neuroepithelium, pineal gland, aorta, testis, heart, and most recently kidney. Patch clamp studies have identified and characterized cGMP-gated cation channels in the cortical collecting duct (CCD) and inner medullary collecting duct (IMCD). cGMP-gated cation channels in kidney share many biophysical and molecular properties with the retinal rod cGMP-gated channel. However, unlike the retinal rod channel, the cGMP-gated cation channel in kidney is inhibited by cGMP and stimulated by increased calcium levels. In the IMCD the cGMP-gated cation channel mediates electrogenic sodium absorption which is inhibited by ANP via cGMP. Recently, cGMP-gated cation channel poly(A+) RNA has been identified in other nephron segments by RT-PCR and in situ PCR hybridization. Furthermore, cGMP-gated cation channel protein has also been identified in all nephron segments by Western blot analysis. These observations suggest that cGMP-gated cation channels, or closely related gene products, may play an important physiological role in all nephron segments. Hormones that increase intracellular cGMP may regulate sodium, and perhaps calcium, uptake in nephron segments proximal to the IMCD. Increases in cell sodium and calcium may regulate other transport and signaling pathways.

AB - Cyclic nucleotide-gated cation channels, which are permeable to monovalent and divalent cations, are expressed in a number of tissues. cDNAs encoding cGMP-gated cation channel subunits have been cloned in retinal rods, cones, olfactory neuroepithelium, pineal gland, aorta, testis, heart, and most recently kidney. Patch clamp studies have identified and characterized cGMP-gated cation channels in the cortical collecting duct (CCD) and inner medullary collecting duct (IMCD). cGMP-gated cation channels in kidney share many biophysical and molecular properties with the retinal rod cGMP-gated channel. However, unlike the retinal rod channel, the cGMP-gated cation channel in kidney is inhibited by cGMP and stimulated by increased calcium levels. In the IMCD the cGMP-gated cation channel mediates electrogenic sodium absorption which is inhibited by ANP via cGMP. Recently, cGMP-gated cation channel poly(A+) RNA has been identified in other nephron segments by RT-PCR and in situ PCR hybridization. Furthermore, cGMP-gated cation channel protein has also been identified in all nephron segments by Western blot analysis. These observations suggest that cGMP-gated cation channels, or closely related gene products, may play an important physiological role in all nephron segments. Hormones that increase intracellular cGMP may regulate sodium, and perhaps calcium, uptake in nephron segments proximal to the IMCD. Increases in cell sodium and calcium may regulate other transport and signaling pathways.

UR - http://www.scopus.com/inward/record.url?scp=0029099586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029099586&partnerID=8YFLogxK

M3 - Article

C2 - 8569074

AN - SCOPUS:0029099586

VL - 48

SP - 1125

EP - 1133

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 4

ER -