The reliability of nipple aspirate and ductal lavage in women at increased risk for breast cancer - A potential tool for breast cancer risk assessment and biomarker evaluation

Kala Visvanathan, D. Santor, S. Z. Ali, A. Brewster, A. Arnold, D. K. Armstrong, N. E. Davidson, K. J. Helzlsouer

Research output: Contribution to journalArticle

Abstract

Purpose: Ductal lavage, a technique used to sample epithelial cells from breast ducts, has potential use in risk assessment and biomarker evaluation among women at increased risk for breast cancer. However, little is known about the reliability of the procedure. Methods: We evaluated the reliability of nipple aspirate (NAF) and ductal lavage at two time points 6 months apart in women at increased risk for breast cancer. Eligible women had a 5-year Gail risk ≥1.66% or lifetime risk of >20%, and/or a family history or personal history of breast cancer. All ducts that produced NAF were cannulated. The κ statistic was used to evaluate reliability of NAF production, cellular yield, and cytologic diagnosis. Results: Sixty-nine women (mean age, 47 years) were enrolled over 35 months. Forty-seven returned for a second visit. At baseline, 65% of premenopausal and 41% of postmenopausal women produced NAF (P = 0.05), of which 72% underwent successful lavage of at least one duct. Samples of inadequate cellular material for diagnosis were significantly more likely in postmenopausal women than in premenopausal women (P = 0.04). Of the women who returned for a second visit, 18 of 24 who produced NAF had at least one duct successfully cannulated. Twenty-four ducts in 14 women were lavaged twice. Among these ducts, cellular yield for the two time points was inconsistent (κ = 0.33 ± 0.13), and only fair cytologic agreement was observed (κ = 0.32 ± 0.15). Ductal lavage was associated with moderate discomfort. Conclusion: Currently, the use of ductal lavage is limited by technical challenges in duct cannulation, inconsistent NAF production, a high rate of inadequate cellular material for diagnosis, fair cytologic reproducibility, and low participant return rates.

Original languageEnglish (US)
Pages (from-to)950-955
Number of pages6
JournalCancer Epidemiology Biomarkers and Prevention
Volume16
Issue number5
DOIs
StatePublished - May 1 2007

    Fingerprint

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this