The relationship of amniotic fluid fluorescence polarization to neonatal lung function

R. L. Cavalieri, J. C. Beck, L. Brand, Wayne A Mitzner, W. T. London, J. W. Johnson

Research output: Contribution to journalArticle

Abstract

Steady-state fluorescence polarization (FP) of 1,6-diphenyl-1,3,5-hexatriene (DPH) in monkey and human amniotic fluid was studied over a wide range of gestational ages. In both of these systems, the fluorescence polarization decreased with advancing gestational age. In the monkey, these measurements were correlated with both biochemical and physiologic parameters of lung function, including maximal lung volume, alveolar stability, percentage of disaturated phosphatidylcholine in lung homogenate, and lecithin/sphingomyelin ratio of amniotic fluid. Fluorescence polarization values correlated well with the lung disaturated phosphatidylcholine content expressed as a percentage of phosphatidylcholine, thus suggesting that the fluorescent probe interacts with a fraction of the amniotic fluid which is closely related to development of the pulmonary surfactant system. Comparison of monkey and human amniotic fluid fluorescence polarizations showed a greater anisotropy of DPH in the monkey fluid at all stages of gestation, thereby indicating a greater microviscosity in monkey pulmonary surfactant.

Original languageEnglish (US)
Pages (from-to)652-657
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume141
Issue number6
StatePublished - 1981
Externally publishedYes

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Fluorescence Polarization
Amniotic Fluid
Haplorhini
Lung
Diphenylhexatriene
Pulmonary Surfactants
Gestational Age
Sphingomyelins
Lecithins
Anisotropy
Phosphatidylcholines
Fluorescent Dyes
Pregnancy

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Cavalieri, R. L., Beck, J. C., Brand, L., Mitzner, W. A., London, W. T., & Johnson, J. W. (1981). The relationship of amniotic fluid fluorescence polarization to neonatal lung function. American Journal of Obstetrics and Gynecology, 141(6), 652-657.

The relationship of amniotic fluid fluorescence polarization to neonatal lung function. / Cavalieri, R. L.; Beck, J. C.; Brand, L.; Mitzner, Wayne A; London, W. T.; Johnson, J. W.

In: American Journal of Obstetrics and Gynecology, Vol. 141, No. 6, 1981, p. 652-657.

Research output: Contribution to journalArticle

Cavalieri, RL, Beck, JC, Brand, L, Mitzner, WA, London, WT & Johnson, JW 1981, 'The relationship of amniotic fluid fluorescence polarization to neonatal lung function', American Journal of Obstetrics and Gynecology, vol. 141, no. 6, pp. 652-657.
Cavalieri, R. L. ; Beck, J. C. ; Brand, L. ; Mitzner, Wayne A ; London, W. T. ; Johnson, J. W. / The relationship of amniotic fluid fluorescence polarization to neonatal lung function. In: American Journal of Obstetrics and Gynecology. 1981 ; Vol. 141, No. 6. pp. 652-657.
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