Abstract
Oxygen radical toxicity has been implacated in the pathogenesis of myocardial reperfusion injury. In the present study we sought to document the existence of a precise temporal relationship between the time course of free radical generation and the time course of alterations of myocardial energy metabolism during early reperfusion. Rabbit hearts perfused within the bore of a 31-Phosphorous NMR spectrometer were subjected to 30 min of total global ischemia at 37°C. At reflow, 12 control hearts received a bolus of normal perfusate and 12 hearts recombinant human superoxide dismutase (h-SOD) as a 60,000 IU bolus followed by a 100 IU/ml infusion for 15 min. Ischemia resulted in similar depletion of tissue ATP and phosphocreatine (PCr) in the two groups. During the first minute of reflow, recovery of PCr was similar in both groups. However, PCr recovery arrested in control hearts after 2 min, at 63% of baseline, and averaged 64 ± 4% after 45 min of reperfusion. In contrast, h-SOD treated hearts recovered 86.7% of baseline PCr content after 2 min, 102% after 10 min of reperfusion (P
Original language | English (US) |
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Pages (from-to) | 1359-1374 |
Number of pages | 16 |
Journal | Journal of Molecular and Cellular Cardiology |
Volume | 23 |
Issue number | 12 |
DOIs | |
State | Published - 1991 |
Keywords
- Electron Paramagentic Resonance
- Nuclear Magnetic Resonance
- Oxygen Free Radicals
- Reperfusion Injury
- Superoxide Dismutase
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine