The relation between inhibition of cell growth and of dihydroorotic acid dehydrogenase by Brequinar Sodium

E. de Kant; H. M. Pinedo; E. Laurensse; G. J. Peters

doi:10.1016/0304-3835(89)90019-0

The relation between inhibition of cell growth and of dihydroorotic acid dehydrogenase by Brequinar Sodium

E. de Kant, H. M. Pinedo, E. Laurensse, G. J. Peters

Research output: Contribution to journal › Article › peer-review

Abstract

The growth inhibitory effects of Breqinar Sodium (DUP-785; NSC 368390) in 7 different cell lines were related to growth rates and to the inhibition of dihydroorotic acid dehydrogenase (DHO-DH) activity. IC50 values were between 0.2 and 5.8 μM; the fastest growing cell line was least sensitive. Despite a large variation in sensitivity, basal activity of DHO-DH showed little variation (only 2-fold) between the different cell lines. Residual activity of DHO-DH in the presence of Brequinar Sodium varied 30-fold. Drug sensitivity correlated with this residual DHO-DH activity; DHO-DH activity was only slightly inhibited by Breqinar Sodium in the most resistant lines, and almost completely in the most sensitive.

Original language	English (US)
Pages (from-to)	123-127
Number of pages	5
Journal	Cancer Letters
Volume	46
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(89)90019-0
State	Published - Jul 15 1989
Externally published	Yes

Keywords

Breqinar Sodium (DUP-785)
dihydroorotic acid dehydrogenase
growth inhibition
pyrimidine de novo

ASJC Scopus subject areas

Cancer Research
Molecular Biology
Oncology

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10.1016/0304-3835(89)90019-0

Cite this

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title = "The relation between inhibition of cell growth and of dihydroorotic acid dehydrogenase by Brequinar Sodium",

abstract = "The growth inhibitory effects of Breqinar Sodium (DUP-785; NSC 368390) in 7 different cell lines were related to growth rates and to the inhibition of dihydroorotic acid dehydrogenase (DHO-DH) activity. IC50 values were between 0.2 and 5.8 μM; the fastest growing cell line was least sensitive. Despite a large variation in sensitivity, basal activity of DHO-DH showed little variation (only 2-fold) between the different cell lines. Residual activity of DHO-DH in the presence of Brequinar Sodium varied 30-fold. Drug sensitivity correlated with this residual DHO-DH activity; DHO-DH activity was only slightly inhibited by Breqinar Sodium in the most resistant lines, and almost completely in the most sensitive.",

keywords = "Breqinar Sodium (DUP-785), dihydroorotic acid dehydrogenase, growth inhibition, pyrimidine de novo",

author = "{de Kant}, E. and Pinedo, {H. M.} and E. Laurensse and Peters, {G. J.}",

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T1 - The relation between inhibition of cell growth and of dihydroorotic acid dehydrogenase by Brequinar Sodium

AU - de Kant, E.

AU - Pinedo, H. M.

AU - Laurensse, E.

AU - Peters, G. J.

PY - 1989/7/15

Y1 - 1989/7/15

N2 - The growth inhibitory effects of Breqinar Sodium (DUP-785; NSC 368390) in 7 different cell lines were related to growth rates and to the inhibition of dihydroorotic acid dehydrogenase (DHO-DH) activity. IC50 values were between 0.2 and 5.8 μM; the fastest growing cell line was least sensitive. Despite a large variation in sensitivity, basal activity of DHO-DH showed little variation (only 2-fold) between the different cell lines. Residual activity of DHO-DH in the presence of Brequinar Sodium varied 30-fold. Drug sensitivity correlated with this residual DHO-DH activity; DHO-DH activity was only slightly inhibited by Breqinar Sodium in the most resistant lines, and almost completely in the most sensitive.

AB - The growth inhibitory effects of Breqinar Sodium (DUP-785; NSC 368390) in 7 different cell lines were related to growth rates and to the inhibition of dihydroorotic acid dehydrogenase (DHO-DH) activity. IC50 values were between 0.2 and 5.8 μM; the fastest growing cell line was least sensitive. Despite a large variation in sensitivity, basal activity of DHO-DH showed little variation (only 2-fold) between the different cell lines. Residual activity of DHO-DH in the presence of Brequinar Sodium varied 30-fold. Drug sensitivity correlated with this residual DHO-DH activity; DHO-DH activity was only slightly inhibited by Breqinar Sodium in the most resistant lines, and almost completely in the most sensitive.

KW - Breqinar Sodium (DUP-785)

KW - dihydroorotic acid dehydrogenase

KW - growth inhibition

KW - pyrimidine de novo

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The relation between inhibition of cell growth and of dihydroorotic acid dehydrogenase by Brequinar Sodium

Abstract

Keywords

ASJC Scopus subject areas

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