The regulation of hypoxia inducible factor-1alpha on osteoblast function in postmenopausal osteoporosis

Xiao dong Liu, Lian fu Deng, Jun Wang, Jin Qi, Q. Zhou, Jin shen Wang, L. Wei, Ya ping Zhu, T. Clemens

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To study the regulation of hypoxia inducible factor-1alpha (HIF-1alpha) on osteoblast function in postmenopausal osteoporosis. METHODS: From October 2004 to May 2006, Cre-Loxp recombinase was used to create mice which the HIF-1alpha gene in osteoblasts was conditional knock-out, 24 female wild-type (WT) mice and 24 female conditional knock-out (CKO) mice of 3 months old were operated on ovariotomy. At 0,4,8 weeks after operation, bone histomorphometry parameters were measured with computer image analysis in HE stain sections and in tetracycline bone double labeling fluorescence sections; Bone density and the trabecular bone architecture parameters were measured by Micro-CT; The mRNA expression of vascular endothelial growth factors (VEGF), RunX2, OC, ALP were detected with quantitative RT-PCR; The protein expression of VEGF and RunX2 were detected with Western-blotting. RESULTS: In CKO mice, the trabecular number, volume, thickness, bone density, mineral apposition rate (MAR), the expression of VEGF, RunX2, OC, ALP on mRNA level and the expression of VEGF, RunX2 on protein level decreased significantly compared with WT mice especially in 8 weeks after operation. CONCLUSIONS: The bone formation ability of osteoblasts in CKO mice was reduced compared with WT mice after ovariotomy. HIF-1alpha can regulate the bone formation ability of osteoblasts in postmenopausal osteoporosis.

Original languageEnglish (US)
Pages (from-to)1274-1278
Number of pages5
JournalZhonghua wai ke za zhi [Chinese journal of surgery]
Volume45
Issue number18
StatePublished - Sep 15 2007
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'The regulation of hypoxia inducible factor-1alpha on osteoblast function in postmenopausal osteoporosis'. Together they form a unique fingerprint.

  • Cite this

    Liu, X. D., Deng, L. F., Wang, J., Qi, J., Zhou, Q., Wang, J. S., Wei, L., Zhu, Y. P., & Clemens, T. (2007). The regulation of hypoxia inducible factor-1alpha on osteoblast function in postmenopausal osteoporosis. Zhonghua wai ke za zhi [Chinese journal of surgery], 45(18), 1274-1278.