The receptor-like tyrosine phosphatase Lar is required for epithelial planar polarity and for axis determination within Drosophila ovarian follicles

H. M. Frydman, A. C. Spradling

Research output: Contribution to journalArticle

Abstract

The follicle cell monolayer that encircles each developing Drosophila oocyte contributes actively to egg development and patterning, and also represents a model stem cell-derived epithelium. We have identified mutations in the receptor-like transmembrane tyrosine phosphatase Lar that disorganize follicle formation, block egg chamber elongation and disrupt Oskar localization, which is an indicator of oocyte anterior-posterior polarity. Alterations in actin filament organization correlate with these defects. Actin filaments in the basal follicle cell domain normally become polarized during stage 6 around the anterior-posterior axis defined by the polar cells, but mutations in Lar frequently disrupt polar cell differentiation and actin polarization. Lar function is only needed in somatic cells, and (for Oskar localization) its action is autonomous to posterior follicle cells. Polarity signals may be laid down by these cells within the extracellular matrix (ECM), possibly in the distribution of the candidate Lar ligand Laminin A, and read out at the time Oskar is localized in a Lar-dependent manner. Lar is not required autonomously to polarize somatic cell actin during stages 6. We show that Lar acts somatically early in oogenesis, during follicle formation, and postulate that it functions in germarium intercyst cells that are required for polar cell specification and differentiation. Our studies suggest that positional information can be stored transiently in the ECM. A major function of Lar may be to transduce such signals.

Original languageEnglish (US)
Pages (from-to)3209-3220
Number of pages12
JournalDevelopment
Volume128
Issue number16
StatePublished - 2001
Externally publishedYes

Fingerprint

Ovarian Follicle
Phosphoric Monoester Hydrolases
Drosophila
Polar Bodies
Actin Cytoskeleton
Oocytes
Extracellular Matrix
Ovum
Actins
Cell Differentiation
Oogenesis
Mutation
tyrosine receptor
Tyrosine
Stem Cells
Epithelium
Ligands

Keywords

  • Actin
  • Axis formation
  • Drosophila
  • Extracellular matrix
  • Follicle
  • Lar
  • Oogenesis
  • Planar polarity

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

The receptor-like tyrosine phosphatase Lar is required for epithelial planar polarity and for axis determination within Drosophila ovarian follicles. / Frydman, H. M.; Spradling, A. C.

In: Development, Vol. 128, No. 16, 2001, p. 3209-3220.

Research output: Contribution to journalArticle

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