The rapid onset of elasticity during the assembly of the bacterial cell-division protein FtsZ

Osigwe Esue, Yiider Tseng, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

FtsZ, a prokaryotic homolog of eukaryotic tubulin, is a major constituent of the bacterial Z-ring, which contracts the cell wall during cell division. Because the mechanical properties of FtsZ are unknown, its function in the maintenance and constriction of the Z-ring is not well understood. Here, quantitative rheometry shows that, at physiological concentrations, FtsZ filaments form, extremely rapidly, highly elastic networks within physiological time scales (∼ minutes), much faster than other major dynamic cytoskeletal filaments, including microtubule, actin, and vimentin in eukaryotes. FtsZ networks display a relatively low viscosity and a high resilience against shear stresses, as well as an elasticity that depends weakly on concentration, G ∼ C0.57, a power-law dependence consistent with crosslinked flexible filaments. Calcium, whose intracellular concentration increases during bacterial division, further enhances the elasticity of FtsZ networks through filament bundling, an effect that occurs in the presence of GTP, not GDP. These studies suggest that FtsZ filaments have the toughness to provide strong mechanical support for the maintenance and circumferential constriction of the bacterial Z-ring.

Original languageEnglish (US)
Pages (from-to)508-516
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume333
Issue number2
DOIs
StatePublished - Jul 29 2005

Fingerprint

Elasticity
Constriction
Cell Division
Cells
Maintenance
Vimentin
Tubulin
Guanosine Triphosphate
Eukaryota
Cytoskeleton
Viscosity
Microtubules
Cell Wall
Toughness
Actins
Shear stress
Proteins
Calcium
Mechanical properties

Keywords

  • Bacterial division
  • FtsZ
  • In vitro
  • Z-ring

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The rapid onset of elasticity during the assembly of the bacterial cell-division protein FtsZ. / Esue, Osigwe; Tseng, Yiider; Wirtz, Denis.

In: Biochemical and Biophysical Research Communications, Vol. 333, No. 2, 29.07.2005, p. 508-516.

Research output: Contribution to journalArticle

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