The rapid H2O2-mediated nonphotodynamic crosslinking of lens crystallins generated by the heme-undecapeptide from cytochrome C: Potential implications for cataractogenesis in man

Richard S. Bodaness, J. Samuel Zigler

Research output: Contribution to journalArticlepeer-review

Abstract

Human cataract lens crystallins are crosslinked and demonstrate a non-tryptophan blue fluorescence. We report here that exposure of lens crystallin to H2O2 within the concentration range reported for human aqueous humor, produces crosslinking of crystallin polypeptides within 10 minutes in the presence of the heme-undecapeptide from cytochrome c. Concomitantly, a blue fluorescence develops. These findings suggest the possibility that under some conditions hydrogen peroxide activation may play a role in cataractogenesis in vivo.

Original languageEnglish (US)
Pages (from-to)592-597
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume113
Issue number2
DOIs
StatePublished - Jun 15 1983

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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