The q-system: A versatile expression system for drosophila

Olena Riabinina, Christopher J. Potter

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Binary expression systems are flexible and versatile genetic tools in Drosophila. The Q-system is a recently developed repressible binary expression system that offers new possibilities for transgene expression and genetic manipulations. In this review chapter, we focus on current state-of-the-art Q-system tools and reagents. We also discuss in vivo applications of the Q-system, together with GAL4/ UAS and LexA/ LexAop systems, for simultaneous expression of multiple effectors, intersectional labeling, and clonal analysis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages53-78
Number of pages26
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1478
ISSN (Print)1064-3745

Keywords

  • Binary expression system
  • Chimeric transactivators
  • GAL4QF
  • Intersectional expression
  • LexAQF
  • MARCM
  • Mitotic recombination
  • Mosaic analysis
  • Neurospora crassa
  • Q-system
  • QF
  • QF2
  • QF2
  • QS
  • QUAS
  • Quinic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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