The Q system: A repressible binary system for transgene expression, lineage tracing, and mosaic analysis

Christopher J. Potter, Bosiljka Tasic, Emilie V. Russler, Liang Liang, Liqun Luo

Research output: Contribution to journalArticle

Abstract

We describe a new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster. This " Q system" offers attractive features for transgene expression in Drosophila and mammalian cells: low basal expression in the absence of the transcriptional activator QF, high QF-induced expression, and QF repression by its repressor QS. Additionally, feeding flies quinic acid can relieve QS repression. The Q system offers many applications, including (1) intersectional " logic gates" with the GAL4 system for manipulating transgene expression patterns, (2) GAL4-independent MARCM analysis, and (3) coupled MARCM analysis to independently visualize and genetically manipulate siblings from any cell division. We demonstrate the utility of the Q system in determining cell division patterns of a neuronal lineage and gene function in cell growth and proliferation, and in dissecting neurons responsible for olfactory attraction. The Q system can be expanded to other uses in Drosophila and to any organism conducive to transgenesis.

Original languageEnglish (US)
Pages (from-to)536-548
Number of pages13
JournalCell
Volume141
Issue number3
DOIs
StatePublished - Apr 1 2010
Externally publishedYes

Keywords

  • DNA
  • Molneuro

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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