The Protein Kinase YakA Regulates G-protein-linked Signaling Responses during Growth and Development of Dictyostelium

Saskia Van Es, Karin E. Weening, Peter N Devreotes

Research output: Contribution to journalArticle

Abstract

A genetic screen for Dictyostelium mutants that phenotypically resemble cells lacking the G-protein β-subunit yielded the protein kinase YakA. Like β-null cells, yakA-null cells fail to enter development and display slow growth on bacterial lawns. We created a temperature-sensitive yakaA mutant and showed that YakA activity is required not only at the onset but also during development. The yakA-null cells have strong defects in folic acid-induced responses, such as actin polymerization and cGMP accumulation, indicating that they play a role in G-protein-mediated signaling responses. We propose that YakA acts downstream of G-proteins, because cAMP receptors still couple to G-proteins in the yakA mutant. In addition, the previously observed growth arrest induced by overexpression of YakA also occurs in gβ mutants. We localized YakA-GFP to the cytosol suggesting that YakA may be a functional homolog of its mammalian counterparts Dyrk2 and Dyrk3, a subclass of dual-specificity Yak-related kinases (Dyrk) with unknown function.

Original languageEnglish (US)
Pages (from-to)30761-30765
Number of pages5
JournalJournal of Biological Chemistry
Volume276
Issue number33
DOIs
StatePublished - Aug 17 2001

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Dictyostelium
Growth and Development
GTP-Binding Proteins
Null Lymphocytes
Protein Kinases
Cyclic AMP Receptors
Protein Subunits
Growth
Folic Acid
Polymerization
Cytosol
Actins
Phosphotransferases
Defects
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Protein Kinase YakA Regulates G-protein-linked Signaling Responses during Growth and Development of Dictyostelium. / Van Es, Saskia; Weening, Karin E.; Devreotes, Peter N.

In: Journal of Biological Chemistry, Vol. 276, No. 33, 17.08.2001, p. 30761-30765.

Research output: Contribution to journalArticle

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