The protective effect of aminoguanidine on erectile function in streptozotocin diabetic rats

Mustafa F. Usta, Trinity Bivalacqua, Dae Yul Yang, Anshiya Ramanitharan, David R. Sell, Ashiwini Viswanathan, Vincent M. Monnier, Wayne J G Hellstrom

Research output: Contribution to journalArticle

Abstract

Purpose: Erectile dysfunction (ED) is frequently associated with diabetes mellitus. We determined if advanced glycation end products (AGEs) are involved in ED and investigated if the selective AGE and inducible nitric oxide synthase (iNOS) inhibitor aminoguanidine (AG) could protect against the development of ED in a diabetic rat model. Materials and Methods: Harlan Sprague-Dawley rats were divided into 3 groups. The 9 nondiabetic rats in group 1 served as age matched controls. Diabetes was induced in the 9 rats in groups 2 and 3, respectively, by intraperitoneal injection of streptozocin (60 mg/kg). While group 2 was given free access to water and a standard diet, group 3 was treated with AG added to drinking water (1 gm/1 daily). Two months after diabetes induction in vivo intracavernous pressure measurements were determined. Penile tissue glycation (furosine on high performance liquid chromatography), AGEs (pentosidine on high performance liquid chromatography and immunohistochemistry), AGE receptor (galectin-3 on immunohistochemistry and Western blot) and iNOS (Western blot) levels were measured in control and diabetic penises. Results: Cavernous tissue furosine, pentosidine, galectin-3 and iNOS protein levels were significantly elevated in the diabetic group compared with controls (p

Original languageEnglish (US)
Pages (from-to)1437-1442
Number of pages6
JournalJournal of Urology
Volume170
Issue number4 I
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

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Nitric Oxide Synthase Type II
Erectile Dysfunction
Streptozocin
Galectin 3
Western Blotting
Immunohistochemistry
High Pressure Liquid Chromatography
Advanced Glycosylation End Products
Penis
Intraperitoneal Injections
Drinking Water
Sprague Dawley Rats
Diabetes Mellitus
Diet
Pressure
Water
pimagedine
Proteins
pentosidine
furosine

Keywords

  • Advanced
  • Diabetes mellitus
  • Glycosylation end products
  • Impotence
  • Penis
  • Rats
  • Sprague-Dawley

ASJC Scopus subject areas

  • Urology

Cite this

Usta, M. F., Bivalacqua, T., Yang, D. Y., Ramanitharan, A., Sell, D. R., Viswanathan, A., ... Hellstrom, W. J. G. (2003). The protective effect of aminoguanidine on erectile function in streptozotocin diabetic rats. Journal of Urology, 170(4 I), 1437-1442. https://doi.org/10.1097/01.ju.0000077557.45582.f3

The protective effect of aminoguanidine on erectile function in streptozotocin diabetic rats. / Usta, Mustafa F.; Bivalacqua, Trinity; Yang, Dae Yul; Ramanitharan, Anshiya; Sell, David R.; Viswanathan, Ashiwini; Monnier, Vincent M.; Hellstrom, Wayne J G.

In: Journal of Urology, Vol. 170, No. 4 I, 01.10.2003, p. 1437-1442.

Research output: Contribution to journalArticle

Usta, MF, Bivalacqua, T, Yang, DY, Ramanitharan, A, Sell, DR, Viswanathan, A, Monnier, VM & Hellstrom, WJG 2003, 'The protective effect of aminoguanidine on erectile function in streptozotocin diabetic rats', Journal of Urology, vol. 170, no. 4 I, pp. 1437-1442. https://doi.org/10.1097/01.ju.0000077557.45582.f3
Usta, Mustafa F. ; Bivalacqua, Trinity ; Yang, Dae Yul ; Ramanitharan, Anshiya ; Sell, David R. ; Viswanathan, Ashiwini ; Monnier, Vincent M. ; Hellstrom, Wayne J G. / The protective effect of aminoguanidine on erectile function in streptozotocin diabetic rats. In: Journal of Urology. 2003 ; Vol. 170, No. 4 I. pp. 1437-1442.
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