TY - JOUR
T1 - The properties and interactions of the isolated α- and β-chains of human haemoglobin
T2 - IV. Immunological studies involving antibodies against the isolated chains
AU - Reichlin, Morris
AU - Bucci, Enrico
AU - Fronticelli, Clara
AU - Wyman, Jeffries
AU - Ioppolo, Eraldo Antonini Carmela
AU - Rossi-Fanelli, Alessandro
PY - 1966
Y1 - 1966
N2 - Antibodies against the isolated α- and β-chains of human haemoglobin produce precipitation and complement fixation with their antigens. Both types of antibody give complement fixation with haemoglobin A, but do not cross-react with the opposite type of chain. The behaviour of the anti α- and anti β-sera is not affected by the presence of p-mercuribenzoate in the immunizing antigen. The reaction of the anti α-serum with free α-chains is the same as with α-chains in native haemoglobin; however, when the accompanying β-chains are replaced by those of another species in hybrids, or by γ-chains in foetal haemoglobin, the strength of the reaction of the anti α-serum is reduced in a specific way. The reaction of anti β-serum with free β-chains is much stronger than when the β-chains are combined with α-chains in native haemoglobin; however, replacement of the α-chains by those of another species in hybrids, or by mutant α-chains in haemoglobin Burlington, has no effect. After anti β-serum has been absorbed by haemoglobin A, it is still capable of giving complement fixation with free β-chains. This shows the presence of an anti β-antibody specific to the free β-chains. The anti α- and anti β-sera are both highly sensitive to even one residue substitution in the chains against which they are directed.
AB - Antibodies against the isolated α- and β-chains of human haemoglobin produce precipitation and complement fixation with their antigens. Both types of antibody give complement fixation with haemoglobin A, but do not cross-react with the opposite type of chain. The behaviour of the anti α- and anti β-sera is not affected by the presence of p-mercuribenzoate in the immunizing antigen. The reaction of the anti α-serum with free α-chains is the same as with α-chains in native haemoglobin; however, when the accompanying β-chains are replaced by those of another species in hybrids, or by γ-chains in foetal haemoglobin, the strength of the reaction of the anti α-serum is reduced in a specific way. The reaction of anti β-serum with free β-chains is much stronger than when the β-chains are combined with α-chains in native haemoglobin; however, replacement of the α-chains by those of another species in hybrids, or by mutant α-chains in haemoglobin Burlington, has no effect. After anti β-serum has been absorbed by haemoglobin A, it is still capable of giving complement fixation with free β-chains. This shows the presence of an anti β-antibody specific to the free β-chains. The anti α- and anti β-sera are both highly sensitive to even one residue substitution in the chains against which they are directed.
KW - PMB
KW - p-mercuribenzoate
UR - http://www.scopus.com/inward/record.url?scp=0013905740&partnerID=8YFLogxK
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U2 - 10.1016/S0022-2836(66)80091-8
DO - 10.1016/S0022-2836(66)80091-8
M3 - Article
C2 - 5961147
AN - SCOPUS:0013905740
SN - 0022-2836
VL - 17
SP - 18
EP - 28
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 1
ER -