The promise of methylation on beads for cancer detection and treatment

Angela A. Guzzetta, Thomas R. Pisanic, Prateek Sharma, Joo Mi Yi, Alejandro Stark, Tza Huei Wang, Nita Ahuja

Research output: Contribution to journalArticle

Abstract

Despite numerous technical hurdles, the realization of true personalized medicine is becoming a progressive reality for the future of patient care. With the development of new techniques and tools to measure the genetic signature of tumors, biomarkers are increasingly being used to detect occult tumors, determine the choice of treatment and predict outcomes. Methylation of CpG islands at the promoter region of genes is a particularly exciting biomarker as it is cancer-specific. Older methods to detect methylation were cumbersome, operator-dependent and required large amounts of DNA. However, a newer technique called methylation on beads has resulted in a more uniform, streamlined and efficient assay. Furthermore, methylation on beads permits the extraction and processing of miniscule amounts of methylated tumor DNA in the peripheral blood. Such a technique may aid in the clinical detection and treatment of cancers in the future.

Original languageEnglish (US)
Pages (from-to)845-852
Number of pages8
JournalExpert Review of Molecular Diagnostics
Volume14
Issue number7
DOIs
StatePublished - 2014

Fingerprint

Methylation
Neoplasms
Precision Medicine
CpG Islands
DNA
Therapeutics
Tumor Biomarkers
Genetic Promoter Regions
Patient Care
Biomarkers
Genes

Keywords

  • biomarker
  • cancer detection
  • colon cancer
  • CpG island methylation
  • methylation on bead
  • pancreatic cancer
  • personalized medicine

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pathology and Forensic Medicine

Cite this

The promise of methylation on beads for cancer detection and treatment. / Guzzetta, Angela A.; Pisanic, Thomas R.; Sharma, Prateek; Yi, Joo Mi; Stark, Alejandro; Wang, Tza Huei; Ahuja, Nita.

In: Expert Review of Molecular Diagnostics, Vol. 14, No. 7, 2014, p. 845-852.

Research output: Contribution to journalArticle

Guzzetta, Angela A. ; Pisanic, Thomas R. ; Sharma, Prateek ; Yi, Joo Mi ; Stark, Alejandro ; Wang, Tza Huei ; Ahuja, Nita. / The promise of methylation on beads for cancer detection and treatment. In: Expert Review of Molecular Diagnostics. 2014 ; Vol. 14, No. 7. pp. 845-852.
@article{8f4f73ac5f4c4f5a98a3fcf76f32b615,
title = "The promise of methylation on beads for cancer detection and treatment",
abstract = "Despite numerous technical hurdles, the realization of true personalized medicine is becoming a progressive reality for the future of patient care. With the development of new techniques and tools to measure the genetic signature of tumors, biomarkers are increasingly being used to detect occult tumors, determine the choice of treatment and predict outcomes. Methylation of CpG islands at the promoter region of genes is a particularly exciting biomarker as it is cancer-specific. Older methods to detect methylation were cumbersome, operator-dependent and required large amounts of DNA. However, a newer technique called methylation on beads has resulted in a more uniform, streamlined and efficient assay. Furthermore, methylation on beads permits the extraction and processing of miniscule amounts of methylated tumor DNA in the peripheral blood. Such a technique may aid in the clinical detection and treatment of cancers in the future.",
keywords = "biomarker, cancer detection, colon cancer, CpG island methylation, methylation on bead, pancreatic cancer, personalized medicine",
author = "Guzzetta, {Angela A.} and Pisanic, {Thomas R.} and Prateek Sharma and Yi, {Joo Mi} and Alejandro Stark and Wang, {Tza Huei} and Nita Ahuja",
year = "2014",
doi = "10.1586/14737159.2014.943665",
language = "English (US)",
volume = "14",
pages = "845--852",
journal = "Expert Review of Anticancer Therapy",
issn = "1473-7140",
publisher = "Expert Reviews Ltd.",
number = "7",

}

TY - JOUR

T1 - The promise of methylation on beads for cancer detection and treatment

AU - Guzzetta, Angela A.

AU - Pisanic, Thomas R.

AU - Sharma, Prateek

AU - Yi, Joo Mi

AU - Stark, Alejandro

AU - Wang, Tza Huei

AU - Ahuja, Nita

PY - 2014

Y1 - 2014

N2 - Despite numerous technical hurdles, the realization of true personalized medicine is becoming a progressive reality for the future of patient care. With the development of new techniques and tools to measure the genetic signature of tumors, biomarkers are increasingly being used to detect occult tumors, determine the choice of treatment and predict outcomes. Methylation of CpG islands at the promoter region of genes is a particularly exciting biomarker as it is cancer-specific. Older methods to detect methylation were cumbersome, operator-dependent and required large amounts of DNA. However, a newer technique called methylation on beads has resulted in a more uniform, streamlined and efficient assay. Furthermore, methylation on beads permits the extraction and processing of miniscule amounts of methylated tumor DNA in the peripheral blood. Such a technique may aid in the clinical detection and treatment of cancers in the future.

AB - Despite numerous technical hurdles, the realization of true personalized medicine is becoming a progressive reality for the future of patient care. With the development of new techniques and tools to measure the genetic signature of tumors, biomarkers are increasingly being used to detect occult tumors, determine the choice of treatment and predict outcomes. Methylation of CpG islands at the promoter region of genes is a particularly exciting biomarker as it is cancer-specific. Older methods to detect methylation were cumbersome, operator-dependent and required large amounts of DNA. However, a newer technique called methylation on beads has resulted in a more uniform, streamlined and efficient assay. Furthermore, methylation on beads permits the extraction and processing of miniscule amounts of methylated tumor DNA in the peripheral blood. Such a technique may aid in the clinical detection and treatment of cancers in the future.

KW - biomarker

KW - cancer detection

KW - colon cancer

KW - CpG island methylation

KW - methylation on bead

KW - pancreatic cancer

KW - personalized medicine

UR - http://www.scopus.com/inward/record.url?scp=84906314747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906314747&partnerID=8YFLogxK

U2 - 10.1586/14737159.2014.943665

DO - 10.1586/14737159.2014.943665

M3 - Article

VL - 14

SP - 845

EP - 852

JO - Expert Review of Anticancer Therapy

JF - Expert Review of Anticancer Therapy

SN - 1473-7140

IS - 7

ER -