The profile of angiogenic factors in vitreous humor of the patients with proliferative diabetic retinopathy

Y. Yu, J. Zhang, R. Zhu, R. Zhao, J. Chen, J. Jin, Y. Tian, S. B. Su

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To explore the expression profile of angiogenic factors associated with proliferative diabetic retinopathy (PDR). Methods: Undiluted vitreous humor samples were obtained from 10 diabetic patients with PDR (10 eyes) and 9 non-diabtic patients (9 eyes). The concentrations of 60 angiogenic factors in the vitreous humor samples were measured by RayBio Angiogenic Cytokine Antibody Array. Some differentially expressed factors were further confirmed in vitreous humor by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the non-diabetic controls, 20 differentially expressed factors with more than 1.50 fold changes were detected in patients with PDR. The median concentration of vascular endothelial growth factor (VEGF), interleukin 6 (IL-6), angiopoietin (ANG)-1, ANG-2, urokinase-type plasminogen activator receptor (uPAR), Follistatin and matrix metalloproteinases 9 (MMP-9) was significantly increased in vitreous samples from PDR compared to controls (P < 0.05). However, (MCP)-1, Angiogenin and Leptin was significantly lower in PDR eyes compared to controls (P < 0.05). In the verification assay using ELISA, ANG-1, ANG-2, IL-6, VEGF, MMP-9, hepatocyte growth factor (HGF) and placenta growth factor (PIGF) concentration were increased in patients with PDR compared to controls (all P-values < 0.05). Conclusion: This is the first report of a comprehensive multiplex analysis to identify angiogenic factors associated with PDR. These angiogenic factors may contribute to the pathogenesis of PDR and may be targets for therapeutic strategies of PDR.

Original languageEnglish (US)
Pages (from-to)280-286
Number of pages7
JournalCurrent Molecular Medicine
Volume17
Issue number4
DOIs
StatePublished - May 1 2017
Externally publishedYes

Keywords

  • Angiogenesis
  • Diabetes
  • ELISA
  • Proliferative diabetic retinopathy
  • Retinopathy
  • Vitreous humor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

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