TY - JOUR
T1 - The profile of angiogenic factors in vitreous humor of the patients with proliferative diabetic retinopathy
AU - Yu, Y.
AU - Zhang, J.
AU - Zhu, R.
AU - Zhao, R.
AU - Chen, J.
AU - Jin, J.
AU - Tian, Y.
AU - Su, S. B.
N1 - Funding Information:
This project was supported in part by the grants from the National Natural Science Foundation of China (31471122) and the grants from the Science and Technology Planning Project of Guangdong Province, China (2014A030313047).
Publisher Copyright:
© 2017 Bentham Science Publishers.
PY - 2017/5/1
Y1 - 2017/5/1
N2 - Objective: To explore the expression profile of angiogenic factors associated with proliferative diabetic retinopathy (PDR). Methods: Undiluted vitreous humor samples were obtained from 10 diabetic patients with PDR (10 eyes) and 9 non-diabtic patients (9 eyes). The concentrations of 60 angiogenic factors in the vitreous humor samples were measured by RayBio Angiogenic Cytokine Antibody Array. Some differentially expressed factors were further confirmed in vitreous humor by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the non-diabetic controls, 20 differentially expressed factors with more than 1.50 fold changes were detected in patients with PDR. The median concentration of vascular endothelial growth factor (VEGF), interleukin 6 (IL-6), angiopoietin (ANG)-1, ANG-2, urokinase-type plasminogen activator receptor (uPAR), Follistatin and matrix metalloproteinases 9 (MMP-9) was significantly increased in vitreous samples from PDR compared to controls (P < 0.05). However, (MCP)-1, Angiogenin and Leptin was significantly lower in PDR eyes compared to controls (P < 0.05). In the verification assay using ELISA, ANG-1, ANG-2, IL-6, VEGF, MMP-9, hepatocyte growth factor (HGF) and placenta growth factor (PIGF) concentration were increased in patients with PDR compared to controls (all P-values < 0.05). Conclusion: This is the first report of a comprehensive multiplex analysis to identify angiogenic factors associated with PDR. These angiogenic factors may contribute to the pathogenesis of PDR and may be targets for therapeutic strategies of PDR.
AB - Objective: To explore the expression profile of angiogenic factors associated with proliferative diabetic retinopathy (PDR). Methods: Undiluted vitreous humor samples were obtained from 10 diabetic patients with PDR (10 eyes) and 9 non-diabtic patients (9 eyes). The concentrations of 60 angiogenic factors in the vitreous humor samples were measured by RayBio Angiogenic Cytokine Antibody Array. Some differentially expressed factors were further confirmed in vitreous humor by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the non-diabetic controls, 20 differentially expressed factors with more than 1.50 fold changes were detected in patients with PDR. The median concentration of vascular endothelial growth factor (VEGF), interleukin 6 (IL-6), angiopoietin (ANG)-1, ANG-2, urokinase-type plasminogen activator receptor (uPAR), Follistatin and matrix metalloproteinases 9 (MMP-9) was significantly increased in vitreous samples from PDR compared to controls (P < 0.05). However, (MCP)-1, Angiogenin and Leptin was significantly lower in PDR eyes compared to controls (P < 0.05). In the verification assay using ELISA, ANG-1, ANG-2, IL-6, VEGF, MMP-9, hepatocyte growth factor (HGF) and placenta growth factor (PIGF) concentration were increased in patients with PDR compared to controls (all P-values < 0.05). Conclusion: This is the first report of a comprehensive multiplex analysis to identify angiogenic factors associated with PDR. These angiogenic factors may contribute to the pathogenesis of PDR and may be targets for therapeutic strategies of PDR.
KW - Angiogenesis
KW - Diabetes
KW - ELISA
KW - Proliferative diabetic retinopathy
KW - Retinopathy
KW - Vitreous humor
UR - http://www.scopus.com/inward/record.url?scp=85040370736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85040370736&partnerID=8YFLogxK
U2 - 10.2174/1566524017666171106111440
DO - 10.2174/1566524017666171106111440
M3 - Article
C2 - 29110608
AN - SCOPUS:85040370736
SN - 1566-5240
VL - 17
SP - 280
EP - 286
JO - Current Molecular Medicine
JF - Current Molecular Medicine
IS - 4
ER -