The prevalence of indoor allergens in the Baltimore area: House dust-mite and animal-dander antigens measured by immunochemical techniques

P. Lind, Philip S. Norman, M. Newton, H. Løwenstein, B. Schwartz

Research output: Contribution to journalArticle

Abstract

Mattress dust samples from 42 homes in Baltimore, Md., were analyzed by ELISA and rocket immunoelectrophoresis (RIE) for major excrement allergens (P1 [equal to Dp 42], Df 6, and Dm 6) of house dust mites, Dermatophagoides pteronyssinus, D. farinae, and D. microceras, by RIE for cat major epithelial allergen (cat Ag I) and horse major epithelial antigen (horse Ag II), and by counter-current immunoelectrophoresis (CCIE) for cat and dog antigens. Dp 42 was detected by ELISA in 24 samples (57%) and Df 6 in 39 samples (92%). All samples were negative to Dm 6. Recorded levels varied from 50 ng of allergen per gram of dust (detection limit) to 15,000 ng/gm. A combined content of Dp 42 and Df 6 >700 ng/gm was associated with a positive reaction in RIE. Concentrations of Dp 42 and Df 6 did not correlate (rS = 0.21; p > 0.05). Cat and dog antigens were frequently detected by CCIE (77% and 63%, respectively). CCIE titer to cat correlated well with quantitation of cat Ag 1 by RIE (p <0.01). Horse antigen was detected in few homes (5%). None of the specific allergen determinations correlated with levels of human serum albumin determined by RIE.

Original languageEnglish (US)
Pages (from-to)541-547
Number of pages7
JournalThe Journal of Allergy and Clinical Immunology
Volume80
Issue number4
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Dander
Pyroglyphidae
Baltimore
Immunoelectrophoresis
Allergens
Antigens
Cats
Horses
Dust
Enzyme-Linked Immunosorbent Assay
Dogs
Dermatophagoides pteronyssinus
Serum Albumin
Limit of Detection

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

The prevalence of indoor allergens in the Baltimore area : House dust-mite and animal-dander antigens measured by immunochemical techniques. / Lind, P.; Norman, Philip S.; Newton, M.; Løwenstein, H.; Schwartz, B.

In: The Journal of Allergy and Clinical Immunology, Vol. 80, No. 4, 1987, p. 541-547.

Research output: Contribution to journalArticle

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