The presence within single K-562 cells of erythropoietic and granulopoietic differentiation markers

J. P. Marie, C. A. Izaguirre, C. I. Civin, J. Mirro, E. A. McCulloch

Research output: Contribution to journalArticlepeer-review

Abstract

The continuous cell line K-562, derived from a patient with CML in blast crisis, was examined for markers of granulopoietic (My-1) and erythropoietic (spectrim) differentiation, using specific antibodies detected by indirect immunofluorescence. Both markers were seen, and in 10%-30% of cells, both were present in the same cells. In contrast, the continuous leukemia line HL-60 and KGl contained My-1 only. Controls consisted of colonies in culture containing both granulopoietic and erythropoietic cells (CFU-GEMM). In these, My-1 was seen only in granulopoietic cells and spectrin in erythropoietic cells. The suggestion is advanced that genes coding for differentiation markers are expressed abnormally in K-562.

Original languageEnglish (US)
Pages (from-to)708-711
Number of pages4
JournalBlood
Volume58
Issue number4
StatePublished - 1981
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

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