TY - JOUR
T1 - The predominance of β (CC) chemokine transcripts in idiopathic inflammatory muscle diseases
AU - Adams, E. M.
AU - Kirkley, J.
AU - Eidelman, G.
AU - Dohlman, J.
AU - Plotz, P. H.
PY - 1997
Y1 - 1997
N2 - Cytokines and chemokines that upregulate major histocompatibility complex class I antigens, recruit lymphocytes, and enhance T-cell-mediated myotoxicity may be important in the pathogenesis of dermatomyositis and polymyositis. We searched for cytokine and chemokine transcripts in inflammatory muscle specimens from 14 newly diagnosed or treated patients. Control specimens from six patients without inflammatory muscle disease were analyzed for transcripts of interleukins-1β, -2, -4, -6, -10, and -15, and interferon-gamma, tumor necrosis factor-α, transforming growth factor-β1, macrophage inflammatory proteins-1α and -1β (MIP-1α, MIP-1β), and the chemokine 'regulated on activation, normally T expressed and secreted' (RANTES). Surprisingly, the proinflammatory and lymphocyte cytokines were detected only sporadically in myositis muscle specimens, and their presence did not correlate with disease activity or treatment status of the patient. In contrast, MIP-1α and MIP-1β were detected in 13 and 6 myositis biopsies, respectively, and RANTES, another β (CC) chemokine, was detected in eight myositis biopsies. This study and other reports of low levels of acute-phase cytokines in myositis patients suggest that the proinflammatory cytokines do not play a major role in ongoing muscle damage. The CC chemokines studied here, in particular MIP-1α, might contribute to ongoing muscle inflammation, and the pathogenesis of inflammation in myositis may follow a previously unrecognized pathway.
AB - Cytokines and chemokines that upregulate major histocompatibility complex class I antigens, recruit lymphocytes, and enhance T-cell-mediated myotoxicity may be important in the pathogenesis of dermatomyositis and polymyositis. We searched for cytokine and chemokine transcripts in inflammatory muscle specimens from 14 newly diagnosed or treated patients. Control specimens from six patients without inflammatory muscle disease were analyzed for transcripts of interleukins-1β, -2, -4, -6, -10, and -15, and interferon-gamma, tumor necrosis factor-α, transforming growth factor-β1, macrophage inflammatory proteins-1α and -1β (MIP-1α, MIP-1β), and the chemokine 'regulated on activation, normally T expressed and secreted' (RANTES). Surprisingly, the proinflammatory and lymphocyte cytokines were detected only sporadically in myositis muscle specimens, and their presence did not correlate with disease activity or treatment status of the patient. In contrast, MIP-1α and MIP-1β were detected in 13 and 6 myositis biopsies, respectively, and RANTES, another β (CC) chemokine, was detected in eight myositis biopsies. This study and other reports of low levels of acute-phase cytokines in myositis patients suggest that the proinflammatory cytokines do not play a major role in ongoing muscle damage. The CC chemokines studied here, in particular MIP-1α, might contribute to ongoing muscle inflammation, and the pathogenesis of inflammation in myositis may follow a previously unrecognized pathway.
KW - cytokines
KW - dermatomyositis
KW - inflammation
KW - polymyositis
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M3 - Article
C2 - 9154644
AN - SCOPUS:0031005292
SN - 1081-650X
VL - 109
SP - 275
EP - 285
JO - Proceedings of the Association of American Physicians
JF - Proceedings of the Association of American Physicians
IS - 3
ER -