The positions of 12 simple sequence repeat markers relative to reference loci on mouse Chromosome 16

Nicholas G. Irving, Michael P. Citron, Roger H Reeves

Research output: Contribution to journalArticle

Abstract

The genetic map positions of 12 simple sequence repeat (SSR) markers spanning mouse Chromosome (Chr) 16 were determined relative to reference markers on that chromosome. Interval mapping data were obtained with a panel of DNAs from two intersubspecific backcrosses. All but one of the markers were typed by use of nonradioactive polymerase chain reaction (PCR) products analyzed on agarose gels. The marker order was determined to be Prm-1, D16Mit9, Igl-1, D16Mit29, D16Mit1/D16Mit2, Smst, D16Mit4, D16Mit11, Gap43, D16Mit14, D16Mit30, D16Mit5, Pit-1, D16Mit27, D16H21S16 (formerly D21S16h), D16Mit19, App, D16Mit7, Sod-1. Two of these markers mapped to the known human Chr 21 (HSA21)/Chr 16 conserved linkage group. Nine additional SSR markers could not be typed because they were not polymorophic (four markers), did not amplify MOLD/Rk DNA (three markers), or failed to give PCR products under a range of conditions (two markers). A subset of the most robust SSRs provide a useful marker set for the analysis of previously unmapped crosses.

Original languageEnglish (US)
Pages (from-to)364-367
Number of pages4
JournalMammalian Genome
Volume4
Issue number7
DOIs
StatePublished - Jul 1993

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Chromosomes, Human, Pair 16
Genetic Markers
Microsatellite Repeats
Chromosomes, Human, Pair 21
Polymerase Chain Reaction
Human Chromosomes
Sepharose
Gels
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

The positions of 12 simple sequence repeat markers relative to reference loci on mouse Chromosome 16. / Irving, Nicholas G.; Citron, Michael P.; Reeves, Roger H.

In: Mammalian Genome, Vol. 4, No. 7, 07.1993, p. 364-367.

Research output: Contribution to journalArticle

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