The plasmodium PHIST and RESA-like protein families of human and rodent malaria parasites

Cristina K. Moreira, Bernina Naissant, Alida Coppi, Brandy L. Bennett, Elena Aime, Blandine Franke-Fayard, Chris J. Janse, Isabelle Coppens, Photini Sinnis, Thomas J. Templeton

Research output: Contribution to journalArticle

Abstract

The phist gene family has members identified across the Plasmodium genus, defined by the presence of a domain of roughly 150 amino acids having conserved aromatic residues and an all alpha-helical structure. The family is highly amplified in P. falciparum, with 65 predicted genes in the genome of the 3D7 isolate. In contrast, in the rodent malaria parasite P. berghei 3 genes are identified, one of which is an apparent pseudogene. Transcripts of the P. berghei phist genes are predominant in schizonts, whereas in P. falciparum transcript profiles span different asexual blood stages and gametocytes. We pursued targeted disruption of P. berghei phist genes in order to characterize a simplistic model for the expanded phist gene repertoire in P. falciparum. Unsuccessful attempts to disrupt P. berghei PBANKA-114540 suggest that this phist gene is essential, while knockout of phist PBANKA-122900 shows an apparent normal progression and non-essential function throughout the life cycle. Epitope-tagging of P. falciparum and P. berghei phist genes confirmed protein export to the erythrocyte cytoplasm and localization with a punctate pattern. Three P. berghei PEXEL/HT-positive exported proteins exhibit at least partial co-localization, in support of a common vesicular compartment in the cytoplasm of erythrocytes infected with rodent malaria parasites.

Original languageEnglish (US)
Article numbere0152510
JournalPLoS One
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Plasmodium
malaria
Malaria
Rodentia
Parasites
rodents
Genes
parasites
Proteins
genes
proteins
Cytoplasm
Erythrocytes
Schizonts
Pseudogenes
Gene Order
Essential Genes
Life Cycle Stages
erythrocytes
cytoplasm

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Moreira, C. K., Naissant, B., Coppi, A., Bennett, B. L., Aime, E., Franke-Fayard, B., ... Templeton, T. J. (2016). The plasmodium PHIST and RESA-like protein families of human and rodent malaria parasites. PLoS One, 11(3), [e0152510]. https://doi.org/10.1371/journal.pone.0152510

The plasmodium PHIST and RESA-like protein families of human and rodent malaria parasites. / Moreira, Cristina K.; Naissant, Bernina; Coppi, Alida; Bennett, Brandy L.; Aime, Elena; Franke-Fayard, Blandine; Janse, Chris J.; Coppens, Isabelle; Sinnis, Photini; Templeton, Thomas J.

In: PLoS One, Vol. 11, No. 3, e0152510, 01.03.2016.

Research output: Contribution to journalArticle

Moreira, CK, Naissant, B, Coppi, A, Bennett, BL, Aime, E, Franke-Fayard, B, Janse, CJ, Coppens, I, Sinnis, P & Templeton, TJ 2016, 'The plasmodium PHIST and RESA-like protein families of human and rodent malaria parasites', PLoS One, vol. 11, no. 3, e0152510. https://doi.org/10.1371/journal.pone.0152510
Moreira CK, Naissant B, Coppi A, Bennett BL, Aime E, Franke-Fayard B et al. The plasmodium PHIST and RESA-like protein families of human and rodent malaria parasites. PLoS One. 2016 Mar 1;11(3). e0152510. https://doi.org/10.1371/journal.pone.0152510
Moreira, Cristina K. ; Naissant, Bernina ; Coppi, Alida ; Bennett, Brandy L. ; Aime, Elena ; Franke-Fayard, Blandine ; Janse, Chris J. ; Coppens, Isabelle ; Sinnis, Photini ; Templeton, Thomas J. / The plasmodium PHIST and RESA-like protein families of human and rodent malaria parasites. In: PLoS One. 2016 ; Vol. 11, No. 3.
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