The plasma proteome identifies expected and novel proteins correlated with micronutrient status in undernourished nepalese children1-3

Robert N Cole, Ingo Ruczinski, Kerry J Schulze, Parul S Christian, Shelley Herbrich, Lee Shu Fune Wu, Lauren R. DeVine, Robert N. O'Meally, Sudeep Shrestha, Tatiana N. Boronina, James D Yager, John Davis Groopman, Keith West

Research output: Contribution to journalArticle

Abstract

Micronutrient deficiencies are common in undernourished societies yet remain inadequately assessed due to the complexity and costs of existing assays. A plasma proteomics-based approach holds promise in quantifying multiple nutrient:protein associations that reflect biological function and nutritional status. To validate this concept, in plasma samples of a cohort of 500 6- to 8-y-old Nepalese children, we estimated cross-sectional correlations between vitamins A (retinol), D (25-hydroxyvitamin D), and E (a-tocopherol), copper, and selenium, measured by conventional assays, and relative abundance of their major plasmabound proteins, measured by quantitative proteomics using 8-plex iTRAQ mass tags. The prevalence of low-to-deficient status was 8.8% (50 children. Employing a linear mixed effectsmodel, we observed the following correlations: retinol:retinol-binding protein 4 (r = 0.88), 25-hydroxyvitamin D:vitamin D-binding protein (r = 0.58), α-tocopherol:apolipoprotein C-III (r = 0.64), copper:ceruloplasmin (r = 0.65), and selenium: selenoprotein P isoform 1 (r = 0.79) (all P <0.0001), passing a false discovery rate threshold of 1% (based on P value-derived q values). Individual proteins explained 34-77% (R2) of variation in their respective nutrient concentration. Adding second proteins to models raised R2 to 48-79%, demonstrating a potential to explain additional variation in nutrient concentration by this strategy. Plasma proteomics can identify and quantify protein biomarkers of micronutrient status in undernourished children. The maternalmicronutrient supplementation trial, fromwhich data were derived as a follow-up activity,was registered at clinicaltrials. gov as NCT00115271. 2013.

Original languageEnglish (US)
Pages (from-to)1540-1548
Number of pages9
JournalJournal of Nutrition
Volume143
Issue number10
DOIs
StatePublished - 2013

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Micronutrients
Proteome
Vitamin A
Proteomics
Tocopherols
Selenium
Proteins
Food
Copper
Selenoprotein P
Vitamin D-Binding Protein
Apolipoprotein C-III
Retinol-Binding Proteins
Ceruloplasmin
Nutritional Status
Protein Isoforms
Biomarkers
Costs and Cost Analysis
25-hydroxyvitamin D

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

The plasma proteome identifies expected and novel proteins correlated with micronutrient status in undernourished nepalese children1-3. / Cole, Robert N; Ruczinski, Ingo; Schulze, Kerry J; Christian, Parul S; Herbrich, Shelley; Wu, Lee Shu Fune; DeVine, Lauren R.; O'Meally, Robert N.; Shrestha, Sudeep; Boronina, Tatiana N.; Yager, James D; Groopman, John Davis; West, Keith.

In: Journal of Nutrition, Vol. 143, No. 10, 2013, p. 1540-1548.

Research output: Contribution to journalArticle

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AU - Herbrich, Shelley

AU - Wu, Lee Shu Fune

AU - DeVine, Lauren R.

AU - O'Meally, Robert N.

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