@article{431f7bb63ef54955b475c3e63b4322c5,
title = "The Pit-1 transcription factor gene is a candidate for the murine Snell dwarf mutation",
abstract = "Two nonallelic mouse mutations with severe dwarf phenotypes are characterized by a lack of growth hormone, prolactin, and thyroid stimulating hormone. The cells that normally synthesize these pituitary hormones express a common transcription factor called GHF-1 or Pit-1. Using an intersubspecific backcross, we have demonstrated tight linkage of the Pit-1 and Snell dwarf (dw) genes on mouse chromosome 16. No recombination was observed between Pit-1 and dw in 110 individuals examined. Southern blot analysis of genomic DNA reveals that the Pit-1 gene is rearranged in C3H HeJ- dw1 dw mice but not in coisogenic +/+ animals, providing molecular evidence that a lesion in the Pit-1 gene results in the Snell dwarf phenotype. Demonstration of low levels of Pit-1 expression in Ames dwarf (df) mice implies that both Pit-1 and df expression may be required for pituitary differentiation.",
author = "Camper, {Sally A.} and Saunders, {Thomas L.} and Katz, {Ronald W.} and Reeves, {Roger H.}",
note = "Funding Information: We thank Dr. Andrzej Bartke for providing dfldf and df/+ mice and for advice on pituitary grafts and husbandry; Dr. Muriel Da-visson for tissues from C3H/HeJ-dwJ mice; the National Hormone Pituitary Program, University of Maryland, Baltimore, MD, for ovine growth hormone; Dr. Miriam Meisler, Dr. Linda Samuelson, and Dr. Francis Collins for reviewing the manuscript; Dr. Shirley Tilghman for encouragement; and Ms. Lori Scarlett for excellent technical assistance. This work was supported in part by a Basil O{\textquoteright}Connor Starter Scholar Grant from the March of Dimes (5-718) (S.A.C.), University of Michigan Rackham School of Graduate Studies and Michigan Memorial-Phoenix Project 2360 and 5550 (S.A.C.),andbyPHSGrantHD22262 (R.H.R.).R.W.K.isapartic-ipant in the Cellular and Biology Training Grant, PHS T32-GM07315.",
year = "1990",
month = nov,
doi = "10.1016/0888-7543(90)90050-5",
language = "English (US)",
volume = "8",
pages = "586--590",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",
}