The phylogeny and active site design of eukaryotic copper-only superoxide dismutases

Ryan L. Peterson, Ahmad Galaleldeen, Johanna Villarreal, Alexander B. Taylor, Diane E. Cabelli, P. John Hart, Valeria L Culotta

Research output: Contribution to journalArticle

Abstract

In eukaryotes the bimetallic Cu/Zn superoxide dismutase (SOD) enzymes play important roles in the biology of reactive oxygen species by disproportionating superoxide anion. Recently, we reported that the fungal pathogen Candida albicans expresses a novel copper-only SOD, known as SOD5, that lacks the zinc cofactor and electrostatic loop (ESL) domain of Cu/Zn-SODs for substrate guidance. Despite these abnormalities, C. albicans SOD5 can disproportionate superoxide at rates limited only by diffusion. Here we demonstrate that this curious copper-only SOD occurs throughout the fungal kingdom as well as in phylogenetically distant oomycetes or "pseudofungi" species. It is the only form of extracellular SOD in fungi and oomycetes, in stark contrast to the extracellular Cu/Zn-SODs of plants and animals. Through structural biology and biochemical approaches we demonstrate that these copper-only SODs have evolved with a specialized active site consisting of two highly conserved residues equivalent to SOD5 Glu-110 and Asp-113. The equivalent positions are zinc binding ligands in Cu/Zn-SODs and have evolved in copper-only SODs to control catalysis and copper binding in lieu of zinc and the ESL. Similar to the zinc ion in Cu/Zn-SODs, SOD5 Glu-110 helps orient a key copper-coordinating histidine and extends the pH range of enzyme catalysis. SOD5 Asp-113 connects to the active site in a manner similar to that of the ESL in Cu/Zn-SODs and assists in copper cofactor binding. Copper-only SODs are virulence factors for certain fungal pathogens; thus this unique active site may be a target for future anti-fungal strategies.

Original languageEnglish (US)
Pages (from-to)20911-20923
Number of pages13
JournalJournal of Biological Chemistry
Volume291
Issue number40
DOIs
StatePublished - Sep 30 2016

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Phylogeny
Superoxide Dismutase
Copper
Catalytic Domain
Static Electricity
Oomycetes
Zinc
Electrostatics
Pathogens
Candida albicans
Catalysis
Superoxides
Candida
Virulence Factors
Enzymes
Eukaryota
Fungi
Reactive Oxygen Species
Animals
Ions

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Peterson, R. L., Galaleldeen, A., Villarreal, J., Taylor, A. B., Cabelli, D. E., Hart, P. J., & Culotta, V. L. (2016). The phylogeny and active site design of eukaryotic copper-only superoxide dismutases. Journal of Biological Chemistry, 291(40), 20911-20923. https://doi.org/10.1074/jbc.M116.748251

The phylogeny and active site design of eukaryotic copper-only superoxide dismutases. / Peterson, Ryan L.; Galaleldeen, Ahmad; Villarreal, Johanna; Taylor, Alexander B.; Cabelli, Diane E.; Hart, P. John; Culotta, Valeria L.

In: Journal of Biological Chemistry, Vol. 291, No. 40, 30.09.2016, p. 20911-20923.

Research output: Contribution to journalArticle

Peterson, RL, Galaleldeen, A, Villarreal, J, Taylor, AB, Cabelli, DE, Hart, PJ & Culotta, VL 2016, 'The phylogeny and active site design of eukaryotic copper-only superoxide dismutases', Journal of Biological Chemistry, vol. 291, no. 40, pp. 20911-20923. https://doi.org/10.1074/jbc.M116.748251
Peterson RL, Galaleldeen A, Villarreal J, Taylor AB, Cabelli DE, Hart PJ et al. The phylogeny and active site design of eukaryotic copper-only superoxide dismutases. Journal of Biological Chemistry. 2016 Sep 30;291(40):20911-20923. https://doi.org/10.1074/jbc.M116.748251
Peterson, Ryan L. ; Galaleldeen, Ahmad ; Villarreal, Johanna ; Taylor, Alexander B. ; Cabelli, Diane E. ; Hart, P. John ; Culotta, Valeria L. / The phylogeny and active site design of eukaryotic copper-only superoxide dismutases. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 40. pp. 20911-20923.
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