The peripheral-type benzodiazepine receptor: a protein of mitochondrial outer membranes utilizing porphyrins as endogenous ligands

Solomon H Snyder, A. Verma, R. R. Trifiletti

Research output: Contribution to journalArticle

Abstract

The peripheral-type benzodiazepine receptor is a site identified by its nanomolar affinity for [3H] diazepam, similar to the affinity of diazepam for the central-type benzodiazepine receptor in the brain. The peripheral-type benzodiazepine receptor occurs in many peripheral tissues but has discrete localizations as indicated by autoradiographic studies showing uniquely high densities of the receptors in the adrenal cortex and in Leydig cells of the testes. Subcellular localization studies reveal a selective association of the receptors with the outer membrane of mitochondria. Photoaffinity labeling of the mitochondrial receptor with [3H] flunitrazepam reveals two discrete labeled protein bands of 30 and 35 kDa, respectively. The 35-kDa band appears to be identical with the voltage-dependent anion channel protein porin. Fractionation of numerous peripheral tissues reveals a single principal endogenous ligand for the receptor, consisting of porphyrins, which display nanomolar affinity. Interactions of porphyrins with the mitochondrial receptor may clarify its physiological role and account for many pharmacological actions of benzodiazepines.

Original languageEnglish (US)
Pages (from-to)282-302
Number of pages21
JournalFASEB Journal
Volume1
Issue number4
StatePublished - 1987

Fingerprint

benzodiazepines
porphyrins
Mitochondrial Proteins
Porphyrins
Mitochondrial Membranes
GABA-A Receptors
Diazepam
Ligands
Membranes
receptors
Voltage-Dependent Anion Channels
Tissue
Flunitrazepam
Porins
Proteins
Mitochondria
proteins
Leydig Cells
Adrenal Cortex
Fractionation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

The peripheral-type benzodiazepine receptor : a protein of mitochondrial outer membranes utilizing porphyrins as endogenous ligands. / Snyder, Solomon H; Verma, A.; Trifiletti, R. R.

In: FASEB Journal, Vol. 1, No. 4, 1987, p. 282-302.

Research output: Contribution to journalArticle

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