The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion

Siân Lax, Debbie L. Hardie, Amy Wilson, Mike R. Douglas, Graham Anderson, David Huso, Clare M. Isacke, Christopher D. Buckley

Research output: Contribution to journalArticle

Abstract

CD248 is a cell surface receptor that specifically identifies fibroblasts and pericytes during development and in association with cancer and inflammation. However, its function is poorly defined and its role in lymphoid organs not studied. Here, we used (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin immunisation and mice lacking CD248 to study whether CD248 modulates popliteal LN (pLN) expansion and subsequent immune responses. We have found that CD248 is required for complete pLN expansion but not for co-ordination of B and T cell compartmentalisation or antibody production following (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin immunisation. In vitro, we show that CD248 expression in human MG63 stromal cells and mouse embryonic fibroblasts leads to a pro-proliferative and pro-migratory phenotype. This correlates with a proliferating CD248+ population observed in vivo during pLN expansion. Taken together, these data highlight a role for CD248 in secondary lymphoid organ remodelling during adaptive immune responses.

Original languageEnglish (US)
Pages (from-to)1884-1889
Number of pages6
JournalEuropean Journal of Immunology
Volume40
Issue number7
DOIs
StatePublished - 2010

Fingerprint

Pericytes
Globulins
Stromal Cells
Chickens
Immunization
Fibroblasts
Lymph Nodes
Cell Surface Receptors
Adaptive Immunity
Antibody Formation
B-Lymphocytes
Inflammation
T-Lymphocytes
Phenotype
Population
Neoplasms
4-hydroxy-5-nitrophenyl acetic acid
In Vitro Techniques

Keywords

  • CD248
  • Endosialin
  • Fibroblast
  • Lymphoid tissue
  • Stromal cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

Cite this

Lax, S., Hardie, D. L., Wilson, A., Douglas, M. R., Anderson, G., Huso, D., ... Buckley, C. D. (2010). The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion. European Journal of Immunology, 40(7), 1884-1889. https://doi.org/10.1002/eji.200939877

The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion. / Lax, Siân; Hardie, Debbie L.; Wilson, Amy; Douglas, Mike R.; Anderson, Graham; Huso, David; Isacke, Clare M.; Buckley, Christopher D.

In: European Journal of Immunology, Vol. 40, No. 7, 2010, p. 1884-1889.

Research output: Contribution to journalArticle

Lax, S, Hardie, DL, Wilson, A, Douglas, MR, Anderson, G, Huso, D, Isacke, CM & Buckley, CD 2010, 'The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion', European Journal of Immunology, vol. 40, no. 7, pp. 1884-1889. https://doi.org/10.1002/eji.200939877
Lax, Siân ; Hardie, Debbie L. ; Wilson, Amy ; Douglas, Mike R. ; Anderson, Graham ; Huso, David ; Isacke, Clare M. ; Buckley, Christopher D. / The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion. In: European Journal of Immunology. 2010 ; Vol. 40, No. 7. pp. 1884-1889.
@article{8f7d2b793e794e7a9fa4a126ff453800,
title = "The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion",
abstract = "CD248 is a cell surface receptor that specifically identifies fibroblasts and pericytes during development and in association with cancer and inflammation. However, its function is poorly defined and its role in lymphoid organs not studied. Here, we used (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin immunisation and mice lacking CD248 to study whether CD248 modulates popliteal LN (pLN) expansion and subsequent immune responses. We have found that CD248 is required for complete pLN expansion but not for co-ordination of B and T cell compartmentalisation or antibody production following (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin immunisation. In vitro, we show that CD248 expression in human MG63 stromal cells and mouse embryonic fibroblasts leads to a pro-proliferative and pro-migratory phenotype. This correlates with a proliferating CD248+ population observed in vivo during pLN expansion. Taken together, these data highlight a role for CD248 in secondary lymphoid organ remodelling during adaptive immune responses.",
keywords = "CD248, Endosialin, Fibroblast, Lymphoid tissue, Stromal cells",
author = "Si{\^a}n Lax and Hardie, {Debbie L.} and Amy Wilson and Douglas, {Mike R.} and Graham Anderson and David Huso and Isacke, {Clare M.} and Buckley, {Christopher D.}",
year = "2010",
doi = "10.1002/eji.200939877",
language = "English (US)",
volume = "40",
pages = "1884--1889",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "7",

}

TY - JOUR

T1 - The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion

AU - Lax, Siân

AU - Hardie, Debbie L.

AU - Wilson, Amy

AU - Douglas, Mike R.

AU - Anderson, Graham

AU - Huso, David

AU - Isacke, Clare M.

AU - Buckley, Christopher D.

PY - 2010

Y1 - 2010

N2 - CD248 is a cell surface receptor that specifically identifies fibroblasts and pericytes during development and in association with cancer and inflammation. However, its function is poorly defined and its role in lymphoid organs not studied. Here, we used (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin immunisation and mice lacking CD248 to study whether CD248 modulates popliteal LN (pLN) expansion and subsequent immune responses. We have found that CD248 is required for complete pLN expansion but not for co-ordination of B and T cell compartmentalisation or antibody production following (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin immunisation. In vitro, we show that CD248 expression in human MG63 stromal cells and mouse embryonic fibroblasts leads to a pro-proliferative and pro-migratory phenotype. This correlates with a proliferating CD248+ population observed in vivo during pLN expansion. Taken together, these data highlight a role for CD248 in secondary lymphoid organ remodelling during adaptive immune responses.

AB - CD248 is a cell surface receptor that specifically identifies fibroblasts and pericytes during development and in association with cancer and inflammation. However, its function is poorly defined and its role in lymphoid organs not studied. Here, we used (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin immunisation and mice lacking CD248 to study whether CD248 modulates popliteal LN (pLN) expansion and subsequent immune responses. We have found that CD248 is required for complete pLN expansion but not for co-ordination of B and T cell compartmentalisation or antibody production following (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin immunisation. In vitro, we show that CD248 expression in human MG63 stromal cells and mouse embryonic fibroblasts leads to a pro-proliferative and pro-migratory phenotype. This correlates with a proliferating CD248+ population observed in vivo during pLN expansion. Taken together, these data highlight a role for CD248 in secondary lymphoid organ remodelling during adaptive immune responses.

KW - CD248

KW - Endosialin

KW - Fibroblast

KW - Lymphoid tissue

KW - Stromal cells

UR - http://www.scopus.com/inward/record.url?scp=77954103111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954103111&partnerID=8YFLogxK

U2 - 10.1002/eji.200939877

DO - 10.1002/eji.200939877

M3 - Article

C2 - 20432232

AN - SCOPUS:77954103111

VL - 40

SP - 1884

EP - 1889

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 7

ER -