The performance of laminin-containing cryogel scaffolds in neural tissue regeneration

Marcin Jurga; Maria B. Dainiak; Anna Sarnowska; Anna Jablonska; Anuj Tripathi; Fatima M. Plieva; Irina N. Savina; Lukasz Strojek; Hans Jungvid; Ashok Kumar; Barbara Lukomska; Krystyna Domanska-Janik; Nico Forraz; Colin P. McGuckin

doi:10.1016/j.biomaterials.2011.01.049

The performance of laminin-containing cryogel scaffolds in neural tissue regeneration

Marcin Jurga, Maria B. Dainiak, Anna Sarnowska, Anna Jablonska, Anuj Tripathi, Fatima M. Plieva, Irina N. Savina, Lukasz Strojek, Hans Jungvid, Ashok Kumar, Barbara Lukomska, Krystyna Domanska-Janik, Nico Forraz, Colin P. McGuckin

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

Currently, there are no effective therapies to restore lost brain neurons, although rapid progress in stem cell biology and biomaterials development provides new tools for regeneration of central nervous system. Here we describe neurogenic properties of bioactive scaffolds generated by cryogelation of dextran or gelatin linked to laminin - the main component of brain extracellular matrix. We showed that such scaffolds promoted differentiation of human cord blood-derived stem cells into artificial neural tissue in vitro. Our experiments revealed that optimal range of scaffolds' pore size for neural tissue engineering was 80-100 microns. We found that scaffold seeded with undifferentiated, but not neutrally committed stem cells, gave optimal cell adhesion and proliferation in " niche" -like structures. Subsequent differentiation resulted in generation of mature 3D networks of neurons (MAP2+) and glia (S100beta+) cells. We showed that cryogel scaffolds could be transplanted into the brain tissue or organotypic hippocampal slices in a rat models. The scaffolds did not induced inflammation mediated by microglial cells (ED1-, Ox43-, Iba1-) and prevented formation of glial scar (GFAP-). Contrary, laminin-rich scaffolds attracted infiltration of host's neuroblasts (NF200+, Nestin+) indicating high neuroregeneration properties.

Original language	English (US)
Pages (from-to)	3423-3434
Number of pages	12
Journal	Biomaterials
Volume	32
Issue number	13
DOIs	https://doi.org/10.1016/j.biomaterials.2011.01.049
State	Published - May 2011
Externally published	Yes

Keywords

Bioactivity
Biocompatibility
Cross-linking
Nerve tissue engineering
Stem cell
Transplantation

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

Access to Document

10.1016/j.biomaterials.2011.01.049

Cite this

Jurga, M., Dainiak, M. B., Sarnowska, A., Jablonska, A., Tripathi, A., Plieva, F. M., Savina, I. N., Strojek, L., Jungvid, H., Kumar, A., Lukomska, B., Domanska-Janik, K., Forraz, N., & McGuckin, C. P. (2011). The performance of laminin-containing cryogel scaffolds in neural tissue regeneration. Biomaterials, 32(13), 3423-3434. https://doi.org/10.1016/j.biomaterials.2011.01.049

Jurga, M, Dainiak, MB, Sarnowska, A, Jablonska, A, Tripathi, A, Plieva, FM, Savina, IN, Strojek, L, Jungvid, H, Kumar, A, Lukomska, B, Domanska-Janik, K, Forraz, N & McGuckin, CP 2011, 'The performance of laminin-containing cryogel scaffolds in neural tissue regeneration', Biomaterials, vol. 32, no. 13, pp. 3423-3434. https://doi.org/10.1016/j.biomaterials.2011.01.049

@article{2fbdf0734f96425a9b75b642ccb54e67,

title = "The performance of laminin-containing cryogel scaffolds in neural tissue regeneration",

abstract = "Currently, there are no effective therapies to restore lost brain neurons, although rapid progress in stem cell biology and biomaterials development provides new tools for regeneration of central nervous system. Here we describe neurogenic properties of bioactive scaffolds generated by cryogelation of dextran or gelatin linked to laminin - the main component of brain extracellular matrix. We showed that such scaffolds promoted differentiation of human cord blood-derived stem cells into artificial neural tissue in vitro. Our experiments revealed that optimal range of scaffolds' pore size for neural tissue engineering was 80-100 microns. We found that scaffold seeded with undifferentiated, but not neutrally committed stem cells, gave optimal cell adhesion and proliferation in {"} niche{"} -like structures. Subsequent differentiation resulted in generation of mature 3D networks of neurons (MAP2+) and glia (S100beta+) cells. We showed that cryogel scaffolds could be transplanted into the brain tissue or organotypic hippocampal slices in a rat models. The scaffolds did not induced inflammation mediated by microglial cells (ED1-, Ox43-, Iba1-) and prevented formation of glial scar (GFAP-). Contrary, laminin-rich scaffolds attracted infiltration of host's neuroblasts (NF200+, Nestin+) indicating high neuroregeneration properties.",

keywords = "Bioactivity, Biocompatibility, Cross-linking, Nerve tissue engineering, Stem cell, Transplantation",

author = "Marcin Jurga and Dainiak, {Maria B.} and Anna Sarnowska and Anna Jablonska and Anuj Tripathi and Plieva, {Fatima M.} and Savina, {Irina N.} and Lukasz Strojek and Hans Jungvid and Ashok Kumar and Barbara Lukomska and Krystyna Domanska-Janik and Nico Forraz and McGuckin, {Colin P.}",

year = "2011",

month = may,

doi = "10.1016/j.biomaterials.2011.01.049",

language = "English (US)",

volume = "32",

pages = "3423--3434",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "13",

}

TY - JOUR

T1 - The performance of laminin-containing cryogel scaffolds in neural tissue regeneration

AU - Jurga, Marcin

AU - Dainiak, Maria B.

AU - Sarnowska, Anna

AU - Jablonska, Anna

AU - Tripathi, Anuj

AU - Plieva, Fatima M.

AU - Savina, Irina N.

AU - Strojek, Lukasz

AU - Jungvid, Hans

AU - Kumar, Ashok

AU - Lukomska, Barbara

AU - Domanska-Janik, Krystyna

AU - Forraz, Nico

AU - McGuckin, Colin P.

PY - 2011/5

Y1 - 2011/5

N2 - Currently, there are no effective therapies to restore lost brain neurons, although rapid progress in stem cell biology and biomaterials development provides new tools for regeneration of central nervous system. Here we describe neurogenic properties of bioactive scaffolds generated by cryogelation of dextran or gelatin linked to laminin - the main component of brain extracellular matrix. We showed that such scaffolds promoted differentiation of human cord blood-derived stem cells into artificial neural tissue in vitro. Our experiments revealed that optimal range of scaffolds' pore size for neural tissue engineering was 80-100 microns. We found that scaffold seeded with undifferentiated, but not neutrally committed stem cells, gave optimal cell adhesion and proliferation in " niche" -like structures. Subsequent differentiation resulted in generation of mature 3D networks of neurons (MAP2+) and glia (S100beta+) cells. We showed that cryogel scaffolds could be transplanted into the brain tissue or organotypic hippocampal slices in a rat models. The scaffolds did not induced inflammation mediated by microglial cells (ED1-, Ox43-, Iba1-) and prevented formation of glial scar (GFAP-). Contrary, laminin-rich scaffolds attracted infiltration of host's neuroblasts (NF200+, Nestin+) indicating high neuroregeneration properties.

AB - Currently, there are no effective therapies to restore lost brain neurons, although rapid progress in stem cell biology and biomaterials development provides new tools for regeneration of central nervous system. Here we describe neurogenic properties of bioactive scaffolds generated by cryogelation of dextran or gelatin linked to laminin - the main component of brain extracellular matrix. We showed that such scaffolds promoted differentiation of human cord blood-derived stem cells into artificial neural tissue in vitro. Our experiments revealed that optimal range of scaffolds' pore size for neural tissue engineering was 80-100 microns. We found that scaffold seeded with undifferentiated, but not neutrally committed stem cells, gave optimal cell adhesion and proliferation in " niche" -like structures. Subsequent differentiation resulted in generation of mature 3D networks of neurons (MAP2+) and glia (S100beta+) cells. We showed that cryogel scaffolds could be transplanted into the brain tissue or organotypic hippocampal slices in a rat models. The scaffolds did not induced inflammation mediated by microglial cells (ED1-, Ox43-, Iba1-) and prevented formation of glial scar (GFAP-). Contrary, laminin-rich scaffolds attracted infiltration of host's neuroblasts (NF200+, Nestin+) indicating high neuroregeneration properties.

KW - Bioactivity

KW - Biocompatibility

KW - Cross-linking

KW - Nerve tissue engineering

KW - Stem cell

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=79952006435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952006435&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2011.01.049

DO - 10.1016/j.biomaterials.2011.01.049

M3 - Article

C2 - 21324403

AN - SCOPUS:79952006435

SN - 0142-9612

VL - 32

SP - 3423

EP - 3434

JO - Biomaterials

JF - Biomaterials

IS - 13

ER -

The performance of laminin-containing cryogel scaffolds in neural tissue regeneration

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this