The pattern of congenital heart defects arising from reduced Tbx5 expression is altered in a Down syndrome mouse model Organogenesis

Renita C. Polk, Peter Gergics, Jeffrey D. Steimle, Huiqing Li, Ivan P. Moskowitz, Sally A. Camper, Roger H. Reeves

Research output: Contribution to journalArticle

Abstract

Background: Nearly half of all individuals with Down Syndrome (DS) have some type of congenital heart defect (CHD), suggesting that DS sensitizes to CHD but does not cause it. We used a common mouse model of DS, the Ts65Dn mouse, to study the contribution of Tbx5, a known modifier of CHD, to heart defects on a trisomic backgroun. Mice that were heterozygous for a Tbx5 null allele were crossed with Ts65Dn mice. Thoraxes of progeny were fixed in 10% formalin, embedded in paraffin, and sectioned for analysis of CHD. Gene expression in embryonic hearts was examined by quantitative PCR and in situ hybridization. A TBX5 DNA binding site was verified by luciferase assays. Methods: Mice that were heterozygous for a Tbx5 null allele were crossed with Ts65Dn mice. Thoraxes of progeny were fixed in 10 % formalin, embedded in paraffin, and sectioned for analysis of CHD. Gene expression in embryonic hearts was examined by quantitative PCR and in situ hybridization. A TBX5 DNA binding site was verified by luciferase assays. Results: We crossed mice that were heterozygous for a Tbx5 null allele with Ts65Dn mice. Mice that were trisomic and carried the Tbx5 mutation (Ts65Dn;Tbx5 +/- ) had a significantly increased incidence of overriding aorta compared to their euploid littermates. Ts65Dn;Tbx5 +/- mice also showed reduced expression of Pitx2, a molecular marker for the left atrium. Transcript levels of the trisomic Adamts1 gene were decreased in Tbx5 +/- mice compared to their euploid littermates. Evidence of a valid binding site for TBX5 upstream of the trisomic Adamts1 locus was also shown. Conclusion: Haploinsufficiency of Tbx5 and trisomy affects alignment of the aorta and this effect may stem from deviations from normal left-right patterning in the heart. We have unveiled a previously unknown interaction between the Tbx5 gene and trisomy, suggesting a connection between Tbx5 and trisomic genes important during heart development.

Original languageEnglish (US)
Article number30
Pages (from-to)4-12
Number of pages9
JournalBMC Developmental Biology
Volume15
Issue number1
DOIs
StatePublished - Jul 25 2015

Keywords

  • Congenital heart defect
  • Down syndrome
  • Heart development
  • Trisomy

ASJC Scopus subject areas

  • Developmental Biology

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