TY - JOUR
T1 - The PAGODAS protocol
T2 - Pediatric assessment group of dengue and Aedes saliva protocol to investigate vector-borne determinants of Aedes-transmitted arboviral infections in Cambodia
AU - Manning, Jessica E.
AU - Oliveira, Fabiano
AU - Parker, Daniel M.
AU - Amaratunga, Chanaki
AU - Kong, Dara
AU - Man, Somnang
AU - Sreng, Sokunthea
AU - Lay, Sreyngim
AU - Nang, Kimsour
AU - Kimsan, Soun
AU - Sokha, Ly
AU - Kamhawi, Shaden
AU - Fay, Michael P.
AU - Suon, Seila
AU - Ruhl, Parker
AU - Ackerman, Hans
AU - Huy, Rekol
AU - Wellems, Thomas E.
AU - Valenzuela, Jesus G.
AU - Leang, Rithea
N1 - Funding Information:
This research was supported by the Intramural Research Programme of the National Institute of Allergy and Infectious Diseases at the National Institutes of Health. Supplemental funding was received from the US-Japan Collaborative Agreement Award.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/20
Y1 - 2018/12/20
N2 - Background: Mosquito-borne arboviruses, like dengue virus, continue to cause significant global morbidity and mortality, particularly in Southeast Asia. When the infectious mosquitoes probe into human skin for a blood meal, they deposit saliva containing a myriad of pharmacologically active compounds, some of which alter the immune response and influence host receptivity to infection, and consequently, the establishment of the virus. Previous reports have highlighted the complexity of mosquito vector-derived factors and immunity in the success of infection. Cumulative evidence from animal models and limited data from humans have identified various vector-derived components, including salivary components, that are co-delivered with the pathogen and play an important role in the dissemination of infection. Much about the roles and effects of these vector-derived factors remain to be discovered. Methods/Design: We describe a longitudinal, pagoda (community)-based pediatric cohort study to evaluate the burden of dengue virus infection and document the immune responses to salivary proteins of Aedes aegypti, the mosquito vector of dengue, Zika, and chikungunya viruses. The study includes community-based seroprevalence assessments in the peri-urban town of Chbar Mon in Kampong Speu Province, Cambodia. The study aims to recruit 771 children between the ages of 2 and 9 years for a three year period of longitudinal follow-up, including twice per year (rainy and dry season) serosurveillance for dengue seroconversion and Ae. aegypti salivary gland homogenate antibody intensity determinations by ELISA assays. Diagnostic tests for acute dengue, Zika and chikungunya viral infections will be performed by RT-PCR. Discussion: This study will serve as a foundation for further understanding of mosquito saliva immunity and its impact on Aedes-transmitted arboviral diseases endemic to Cambodia. Trial registration: NCT03534245 registered on 23 May 2018.
AB - Background: Mosquito-borne arboviruses, like dengue virus, continue to cause significant global morbidity and mortality, particularly in Southeast Asia. When the infectious mosquitoes probe into human skin for a blood meal, they deposit saliva containing a myriad of pharmacologically active compounds, some of which alter the immune response and influence host receptivity to infection, and consequently, the establishment of the virus. Previous reports have highlighted the complexity of mosquito vector-derived factors and immunity in the success of infection. Cumulative evidence from animal models and limited data from humans have identified various vector-derived components, including salivary components, that are co-delivered with the pathogen and play an important role in the dissemination of infection. Much about the roles and effects of these vector-derived factors remain to be discovered. Methods/Design: We describe a longitudinal, pagoda (community)-based pediatric cohort study to evaluate the burden of dengue virus infection and document the immune responses to salivary proteins of Aedes aegypti, the mosquito vector of dengue, Zika, and chikungunya viruses. The study includes community-based seroprevalence assessments in the peri-urban town of Chbar Mon in Kampong Speu Province, Cambodia. The study aims to recruit 771 children between the ages of 2 and 9 years for a three year period of longitudinal follow-up, including twice per year (rainy and dry season) serosurveillance for dengue seroconversion and Ae. aegypti salivary gland homogenate antibody intensity determinations by ELISA assays. Diagnostic tests for acute dengue, Zika and chikungunya viral infections will be performed by RT-PCR. Discussion: This study will serve as a foundation for further understanding of mosquito saliva immunity and its impact on Aedes-transmitted arboviral diseases endemic to Cambodia. Trial registration: NCT03534245 registered on 23 May 2018.
KW - Arbovirus
KW - Cohort
KW - Mosquito
KW - Saliva
KW - Seroprevalence
KW - Vaccines
KW - Vector
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U2 - 10.1186/s13071-018-3224-7
DO - 10.1186/s13071-018-3224-7
M3 - Article
C2 - 30572920
AN - SCOPUS:85058918486
VL - 11
JO - Parasites and Vectors
JF - Parasites and Vectors
SN - 1756-3305
IS - 1
M1 - 664
ER -