The Paf1 complex promotes displacement of histones upon rapid induction of transcription by RNA polymerase II

Heather A. Marton, Stephen Desiderio

Research output: Contribution to journalArticle

Abstract

Background: The yeast Paf1 protein complex is required for efficient transcription elongation by RNA polymerase II (RNA pol II), but the precise role of the complex has been unclear. Results: Here we show that depletion of the Ctr9 or Paf1 component of the Paf1 complex delays the loss of histones from the GAL1 gene upon induction. This delay in histone removal is accompanied by a decrease in association of RNA pol II with GAL1 and altered distribution of the polymerase along the locus. Conclusion: These observations may explain why initial induction of GAL transcripts is reduced in Ctr9- or Paf1-deficient cells, and is consistent with a model suggesting that the Paf1 complex and the histone modifications that it mediates increase efficiency of transcriptional elongation by promoting nucleosomal destabilization and histone removal.

Original languageEnglish (US)
Article number4
JournalBMC Molecular Biology
Volume9
DOIs
StatePublished - Jan 14 2008

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RNA Polymerase II
Transcription
Histones
Histone Code
Elongation
Fungal Proteins
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

The Paf1 complex promotes displacement of histones upon rapid induction of transcription by RNA polymerase II. / Marton, Heather A.; Desiderio, Stephen.

In: BMC Molecular Biology, Vol. 9, 4, 14.01.2008.

Research output: Contribution to journalArticle

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