The P Granules of C. elegans: A Genetic Model for the Study of RNA–Protein Condensates

Research output: Contribution to journalArticle

Abstract

P granules are RNA/protein condensates in the germline of Caenorhabditis elegans. Genetic analyses have begun to identify the proteins that regulate P granule assembly in the cytoplasm of zygotes. Among them, the RGG-domain protein PGL-3, the intrinsically disordered protein MEG-3, and the RNA helicase LAF-1 all bind and phase separate with RNA in vitro. We discuss how RNA-induced phase separation, competition with other RNA-binding proteins, and reversible phosphorylation contribute to the asymmetric localization of P granules in the cytoplasm of newly fertilized embryos. P granules contain RNA silencing complexes that monitor the germline transcriptome and may provide an RNA memory of germline gene expression across generations.

Original languageEnglish (US)
JournalJournal of Molecular Biology
DOIs
StateAccepted/In press - Jan 1 2018

Keywords

  • C. elegans
  • Germline condensates
  • P granules
  • RNA granules

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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