The osteoprotective effect of psoralen in ovariectomy-induced osteoporotic rats via stimulating the osteoblastic differentiation from bone mesenchymal stem cells

Zhu Yang, Jian Hua Huang, Shu Fen Liu, Yong Jian Zhao, Zi Yin Shen, Yong Jun Wang, Qin Bian

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: Psoralea corylifolia extract has been reported to promote bone formation in osteoporotic animals. Psoralen (PSO), a flavonoid glycoside, as the active component of P corylifolia L, is effective in increasing new bone-forming osteoblasts in parietal bone defects. However, the effect and molecular mechanisms of PSO on bone mesenchymal stem cells (bMSCs) in the osteoporotic state are widely unknown. This study was designed to evaluate the osteoprotective effect of PSO in ovariectomy (OVX)-induced rats and to seek possible molecular mechanisms of PSO in bMSCs. METHODS: We observed the osteogenic effect of PSO (3-month treatment) on osteoporotic rat models induced by OVX via testing bone densitometry, histomorphometries, and immunohistochemistry in vivo. Alkaline phosphatase staining and colony-forming unit-fibroblast and colony-forming unit-adipocyte assays were performed to evaluate the differentiation potential of bMSCs ex vivo. In addition, the molecular targets of PSO in bMSCs were detected by stem cell microarray analysis of 256 genes and confirmed by real-time reverse transcription-polymerase chain reaction. RESULTS: Micro-CT morphometry analysis showed that PSO significantly improved bone mass indicators including increased trabecular thickness and decreased trabecular space. Meanwhile, PSO elevated the well-known osteogenic marker osteocalcin level in OVX-induced osteoporotic rats. Next, in ex vivo studies, we revealed that PSO facilitated alkaline phosphatase staining and increased the colony-forming unit-fibroblasts. Based on gene expression profile analysis, we screened a set of genes dysregulated in OVX but reversed by PSO treatment. These genes were highly enriched in the Notch signaling pathway, which was documented to play a role in bMSC differentiation. CONCLUSIONS: Our findings show that PSO promotes bone mass in OVX-induced osteoporotic rats. This effect of PSO is highly related to the stimulation of differentiation of bMSCs to osteoblasts.

Original languageEnglish (US)
Pages (from-to)1156-1164
Number of pages9
JournalMenopause
Volume19
Issue number10
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

Fingerprint

Ficusin
Ovariectomy
Mesenchymal Stromal Cells
Bone and Bones
Stem Cells
Osteoblasts
Alkaline Phosphatase
Psoralea
Fibroblasts
Colony-Forming Units Assay
Tissue Array Analysis
Parietal Bone
Staining and Labeling
Genes
Densitometry
Osteocalcin
Glycosides
Transcriptome
Flavonoids
Adipocytes

Keywords

  • Bone mesenchymal stem cell
  • Osteoporosis
  • Ovariectomy
  • Psoralen
  • Stem cell microarray

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

The osteoprotective effect of psoralen in ovariectomy-induced osteoporotic rats via stimulating the osteoblastic differentiation from bone mesenchymal stem cells. / Yang, Zhu; Huang, Jian Hua; Liu, Shu Fen; Zhao, Yong Jian; Shen, Zi Yin; Wang, Yong Jun; Bian, Qin.

In: Menopause, Vol. 19, No. 10, 01.10.2012, p. 1156-1164.

Research output: Contribution to journalArticle

Yang, Zhu ; Huang, Jian Hua ; Liu, Shu Fen ; Zhao, Yong Jian ; Shen, Zi Yin ; Wang, Yong Jun ; Bian, Qin. / The osteoprotective effect of psoralen in ovariectomy-induced osteoporotic rats via stimulating the osteoblastic differentiation from bone mesenchymal stem cells. In: Menopause. 2012 ; Vol. 19, No. 10. pp. 1156-1164.
@article{4a0b80d0fe2943378b29a7bc06bee5ba,
title = "The osteoprotective effect of psoralen in ovariectomy-induced osteoporotic rats via stimulating the osteoblastic differentiation from bone mesenchymal stem cells",
abstract = "OBJECTIVE: Psoralea corylifolia extract has been reported to promote bone formation in osteoporotic animals. Psoralen (PSO), a flavonoid glycoside, as the active component of P corylifolia L, is effective in increasing new bone-forming osteoblasts in parietal bone defects. However, the effect and molecular mechanisms of PSO on bone mesenchymal stem cells (bMSCs) in the osteoporotic state are widely unknown. This study was designed to evaluate the osteoprotective effect of PSO in ovariectomy (OVX)-induced rats and to seek possible molecular mechanisms of PSO in bMSCs. METHODS: We observed the osteogenic effect of PSO (3-month treatment) on osteoporotic rat models induced by OVX via testing bone densitometry, histomorphometries, and immunohistochemistry in vivo. Alkaline phosphatase staining and colony-forming unit-fibroblast and colony-forming unit-adipocyte assays were performed to evaluate the differentiation potential of bMSCs ex vivo. In addition, the molecular targets of PSO in bMSCs were detected by stem cell microarray analysis of 256 genes and confirmed by real-time reverse transcription-polymerase chain reaction. RESULTS: Micro-CT morphometry analysis showed that PSO significantly improved bone mass indicators including increased trabecular thickness and decreased trabecular space. Meanwhile, PSO elevated the well-known osteogenic marker osteocalcin level in OVX-induced osteoporotic rats. Next, in ex vivo studies, we revealed that PSO facilitated alkaline phosphatase staining and increased the colony-forming unit-fibroblasts. Based on gene expression profile analysis, we screened a set of genes dysregulated in OVX but reversed by PSO treatment. These genes were highly enriched in the Notch signaling pathway, which was documented to play a role in bMSC differentiation. CONCLUSIONS: Our findings show that PSO promotes bone mass in OVX-induced osteoporotic rats. This effect of PSO is highly related to the stimulation of differentiation of bMSCs to osteoblasts.",
keywords = "Bone mesenchymal stem cell, Osteoporosis, Ovariectomy, Psoralen, Stem cell microarray",
author = "Zhu Yang and Huang, {Jian Hua} and Liu, {Shu Fen} and Zhao, {Yong Jian} and Shen, {Zi Yin} and Wang, {Yong Jun} and Qin Bian",
year = "2012",
month = "10",
day = "1",
doi = "10.1097/gme.0b013e3182507e18",
language = "English (US)",
volume = "19",
pages = "1156--1164",
journal = "Menopause",
issn = "1072-3714",
publisher = "Lippincott Williams and Wilkins",
number = "10",

}

TY - JOUR

T1 - The osteoprotective effect of psoralen in ovariectomy-induced osteoporotic rats via stimulating the osteoblastic differentiation from bone mesenchymal stem cells

AU - Yang, Zhu

AU - Huang, Jian Hua

AU - Liu, Shu Fen

AU - Zhao, Yong Jian

AU - Shen, Zi Yin

AU - Wang, Yong Jun

AU - Bian, Qin

PY - 2012/10/1

Y1 - 2012/10/1

N2 - OBJECTIVE: Psoralea corylifolia extract has been reported to promote bone formation in osteoporotic animals. Psoralen (PSO), a flavonoid glycoside, as the active component of P corylifolia L, is effective in increasing new bone-forming osteoblasts in parietal bone defects. However, the effect and molecular mechanisms of PSO on bone mesenchymal stem cells (bMSCs) in the osteoporotic state are widely unknown. This study was designed to evaluate the osteoprotective effect of PSO in ovariectomy (OVX)-induced rats and to seek possible molecular mechanisms of PSO in bMSCs. METHODS: We observed the osteogenic effect of PSO (3-month treatment) on osteoporotic rat models induced by OVX via testing bone densitometry, histomorphometries, and immunohistochemistry in vivo. Alkaline phosphatase staining and colony-forming unit-fibroblast and colony-forming unit-adipocyte assays were performed to evaluate the differentiation potential of bMSCs ex vivo. In addition, the molecular targets of PSO in bMSCs were detected by stem cell microarray analysis of 256 genes and confirmed by real-time reverse transcription-polymerase chain reaction. RESULTS: Micro-CT morphometry analysis showed that PSO significantly improved bone mass indicators including increased trabecular thickness and decreased trabecular space. Meanwhile, PSO elevated the well-known osteogenic marker osteocalcin level in OVX-induced osteoporotic rats. Next, in ex vivo studies, we revealed that PSO facilitated alkaline phosphatase staining and increased the colony-forming unit-fibroblasts. Based on gene expression profile analysis, we screened a set of genes dysregulated in OVX but reversed by PSO treatment. These genes were highly enriched in the Notch signaling pathway, which was documented to play a role in bMSC differentiation. CONCLUSIONS: Our findings show that PSO promotes bone mass in OVX-induced osteoporotic rats. This effect of PSO is highly related to the stimulation of differentiation of bMSCs to osteoblasts.

AB - OBJECTIVE: Psoralea corylifolia extract has been reported to promote bone formation in osteoporotic animals. Psoralen (PSO), a flavonoid glycoside, as the active component of P corylifolia L, is effective in increasing new bone-forming osteoblasts in parietal bone defects. However, the effect and molecular mechanisms of PSO on bone mesenchymal stem cells (bMSCs) in the osteoporotic state are widely unknown. This study was designed to evaluate the osteoprotective effect of PSO in ovariectomy (OVX)-induced rats and to seek possible molecular mechanisms of PSO in bMSCs. METHODS: We observed the osteogenic effect of PSO (3-month treatment) on osteoporotic rat models induced by OVX via testing bone densitometry, histomorphometries, and immunohistochemistry in vivo. Alkaline phosphatase staining and colony-forming unit-fibroblast and colony-forming unit-adipocyte assays were performed to evaluate the differentiation potential of bMSCs ex vivo. In addition, the molecular targets of PSO in bMSCs were detected by stem cell microarray analysis of 256 genes and confirmed by real-time reverse transcription-polymerase chain reaction. RESULTS: Micro-CT morphometry analysis showed that PSO significantly improved bone mass indicators including increased trabecular thickness and decreased trabecular space. Meanwhile, PSO elevated the well-known osteogenic marker osteocalcin level in OVX-induced osteoporotic rats. Next, in ex vivo studies, we revealed that PSO facilitated alkaline phosphatase staining and increased the colony-forming unit-fibroblasts. Based on gene expression profile analysis, we screened a set of genes dysregulated in OVX but reversed by PSO treatment. These genes were highly enriched in the Notch signaling pathway, which was documented to play a role in bMSC differentiation. CONCLUSIONS: Our findings show that PSO promotes bone mass in OVX-induced osteoporotic rats. This effect of PSO is highly related to the stimulation of differentiation of bMSCs to osteoblasts.

KW - Bone mesenchymal stem cell

KW - Osteoporosis

KW - Ovariectomy

KW - Psoralen

KW - Stem cell microarray

UR - http://www.scopus.com/inward/record.url?scp=84867010021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867010021&partnerID=8YFLogxK

U2 - 10.1097/gme.0b013e3182507e18

DO - 10.1097/gme.0b013e3182507e18

M3 - Article

C2 - 22781784

AN - SCOPUS:84867010021

VL - 19

SP - 1156

EP - 1164

JO - Menopause

JF - Menopause

SN - 1072-3714

IS - 10

ER -