The orphan nuclear receptor DHR38 influences transcription of the DOPA decarboxylase gene in epidermal and neural tissues of Drosophila melanogaster

Arthur Hilliker, Monica M. Davis, Ping Yang, Liam Lucian Chen, Sandra L. O'Keefe, Ross B. Hodgetts

Research output: Contribution to journalArticle

Abstract

The DOPA decarboxylase gene (Ddc) belongs to the "early-late" class of ecdysone-inducible genes in Drosophila melanogaster. Its expression is up-regulated in epidermal tissues by the ecdysone receptor acting through a response element, EcRE. In this paper, we show that another member of the nuclear receptor superfamily, DHR38, may act as a repressor of epidermal Ddc while inducing Ddc expression in neuronal cells. DHR38 does not behave as a classical co-repressor of the ecdysone receptor though, since the site through which DHR38 acts is distinct from the EcRE. Ectopic expression of a Dhr38 cDNA from a heat-shock promoter completely repressed transcription from the endogenous Ddc promoter and from an intact reporter construct in the hypoderm and in imaginal discs. Ectopic DHR38 had no effect on the transcription of a reporter driven by a Ddc fragment missing the DHR38 binding site. Neither reporter expression nor endogenous Ddc transcript levels were affected in a Dhr38 mutant background. Because most mutant organisms pupariate apparently normally and many of these survive to eclose, we believe that some functional redundancy exists within the Dhr38 regulatory network operating in epidermal tissues. In contrast to its apparent repressor function in epidermal tissues, DHR38 may act as a positive regulator of neural Ddc expression. Ectopic expression of DHR38 throughout the CNS induced as much as a 20-fold increase in Ddc transcripts in the set of neurons in which DDC normally appears.

Original languageEnglish (US)
Pages (from-to)1049-1060
Number of pages12
JournalGenome
Volume50
Issue number11
DOIs
StatePublished - Nov 2007
Externally publishedYes

Fingerprint

Orphan Nuclear Receptors
Carboxy-Lyases
Drosophila melanogaster
nerve tissue
transcription (genetics)
receptors
Genes
genes
neurons
promoter regions
imaginal discs
gene expression
mutants
Imaginal Discs
ecdysone
Ecdysone
Gene Expression
response elements
Co-Repressor Proteins
Response Elements

Keywords

  • Ddc
  • Dhr38
  • Drosophila melanogaster
  • NGFI
  • Orphan nuclear receptor

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Molecular Biology
  • Agricultural and Biological Sciences(all)
  • Genetics(clinical)

Cite this

The orphan nuclear receptor DHR38 influences transcription of the DOPA decarboxylase gene in epidermal and neural tissues of Drosophila melanogaster. / Hilliker, Arthur; Davis, Monica M.; Yang, Ping; Chen, Liam Lucian; O'Keefe, Sandra L.; Hodgetts, Ross B.

In: Genome, Vol. 50, No. 11, 11.2007, p. 1049-1060.

Research output: Contribution to journalArticle

Hilliker, Arthur ; Davis, Monica M. ; Yang, Ping ; Chen, Liam Lucian ; O'Keefe, Sandra L. ; Hodgetts, Ross B. / The orphan nuclear receptor DHR38 influences transcription of the DOPA decarboxylase gene in epidermal and neural tissues of Drosophila melanogaster. In: Genome. 2007 ; Vol. 50, No. 11. pp. 1049-1060.
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