The organization of histones and DNA in chromatin: Evidence for an arginine-rich histone kernel

Rafael D. Camerini-Otero, Barbara Sollner-Webb, Gary Felsenfeld

Research output: Contribution to journalArticle

Abstract

We have examined the role played by various histones in the organization of the DNA of the nucleosome, using staphylococcal nuclease as a probe of DNA conformation. When this enzyme attacks chromatin, a series of fragments evenly spaced at 10 base pair intervals is generated, reflecting the histone-DNA interactions within the nucleosome structure. To determine what contribution the various histones make to DNA organization, we have studied the staphylococcal nuclease digestion patterns of complexes of DNA with purified histones. Virtually all possible combinations of homogeneous histones were reconstituted onto DNA. Exhaustive digestion of a complex containing the four histones H2A, H2B, H3, and H4 yields a DNA fragment pattern very similar to that of whole chromatin. The only other combinations of histones capable of inducing chromatin-like DNA organization are H2A/H2B/H4 and those mixtures containing both H3 and H4. From an examination of the kinetics of digestion of H3/H4 reconstitutes, we conclude that although the other histones have a role in DNA organization within the nucleosome, the arginine-rich histone pair, H3/H4, can organize DNA segments the length of the nucleosome core in the absence of all other histones.

Original languageEnglish (US)
Pages (from-to)333-347
Number of pages15
JournalCell
Volume8
Issue number3
DOIs
StatePublished - 1976
Externally publishedYes

Fingerprint

Histones
Chromatin
Arginine
DNA
Nucleosomes
Micrococcal Nuclease
Digestion
Nucleic Acid Conformation
DNA Probes
Base Pairing
Conformations
Kinetics
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

The organization of histones and DNA in chromatin : Evidence for an arginine-rich histone kernel. / Camerini-Otero, Rafael D.; Sollner-Webb, Barbara; Felsenfeld, Gary.

In: Cell, Vol. 8, No. 3, 1976, p. 333-347.

Research output: Contribution to journalArticle

Camerini-Otero, Rafael D. ; Sollner-Webb, Barbara ; Felsenfeld, Gary. / The organization of histones and DNA in chromatin : Evidence for an arginine-rich histone kernel. In: Cell. 1976 ; Vol. 8, No. 3. pp. 333-347.
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