The onset of fermentative metabolism in continuous cultures depends on the catabolite repression properties of Saccharomyces cerevisiae

Sonia Cortassa, Miguel A. Aon

Research output: Contribution to journalArticle

Abstract

In glucose-limited continuous cultures, a Crabtree positive yeast such as Saccharomyces cerevisiae displays respiratory metabolism at low dilution rates (D) and respirofermentative metabolism at high D. We hypothesized that the onset of fermentative metabolism is related with the catabolite repression or glucose repression effect. To test this hypothesis, we have investigated the physiological behaviour in glucose-limited continuous cultures of S.cerevisiae strain CEN.PK122 and isogenic mutants, snf1 (cat1) and snf4 (cat3), defective in proteins involved in the release from glucose repression and the mutant in glucose repression mig1. We analyzed the behaviour of the wild type and mutant strains at steady state in chemostat cultures as a function of D. Wild-type cells displayed respiratory metabolism up to a D of 0.2 h-1. snf1 and snf4 mutants started fermenting after a D of 0.1 and 0.15 h-1, respectively. The latter behavior was not due to an impairment of respiration since their specific rate of oxygen consumption was similar or even higher than that shown by the wild type and the other mutants in the whole range of D studied. We conclude that the onset of fermentative metabolism in yeast growing in chemostat cultures is related with glucose repression.

Original languageEnglish (US)
Pages (from-to)705-712
Number of pages8
JournalEnzyme and Microbial Technology
Volume22
Issue number8
DOIs
StatePublished - Jun 1998
Externally publishedYes

Fingerprint

Catabolite Repression
Metabolism
Yeast
Glucose
Saccharomyces cerevisiae
Chemostats
Yeasts
Oxygen Consumption
Dilution
Respiration
Oxygen
Proteins

Keywords

  • Catabolite repression mutants
  • Chemostat cultures
  • Fermentation
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

The onset of fermentative metabolism in continuous cultures depends on the catabolite repression properties of Saccharomyces cerevisiae. / Cortassa, Sonia; Aon, Miguel A.

In: Enzyme and Microbial Technology, Vol. 22, No. 8, 06.1998, p. 705-712.

Research output: Contribution to journalArticle

@article{1483cc97438c4a97abf88a9592445ecc,
title = "The onset of fermentative metabolism in continuous cultures depends on the catabolite repression properties of Saccharomyces cerevisiae",
abstract = "In glucose-limited continuous cultures, a Crabtree positive yeast such as Saccharomyces cerevisiae displays respiratory metabolism at low dilution rates (D) and respirofermentative metabolism at high D. We hypothesized that the onset of fermentative metabolism is related with the catabolite repression or glucose repression effect. To test this hypothesis, we have investigated the physiological behaviour in glucose-limited continuous cultures of S.cerevisiae strain CEN.PK122 and isogenic mutants, snf1 (cat1) and snf4 (cat3), defective in proteins involved in the release from glucose repression and the mutant in glucose repression mig1. We analyzed the behaviour of the wild type and mutant strains at steady state in chemostat cultures as a function of D. Wild-type cells displayed respiratory metabolism up to a D of 0.2 h-1. snf1 and snf4 mutants started fermenting after a D of 0.1 and 0.15 h-1, respectively. The latter behavior was not due to an impairment of respiration since their specific rate of oxygen consumption was similar or even higher than that shown by the wild type and the other mutants in the whole range of D studied. We conclude that the onset of fermentative metabolism in yeast growing in chemostat cultures is related with glucose repression.",
keywords = "Catabolite repression mutants, Chemostat cultures, Fermentation, Saccharomyces cerevisiae",
author = "Sonia Cortassa and Aon, {Miguel A.}",
year = "1998",
month = "6",
doi = "10.1016/S0141-0229(97)00268-8",
language = "English (US)",
volume = "22",
pages = "705--712",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier Inc.",
number = "8",

}

TY - JOUR

T1 - The onset of fermentative metabolism in continuous cultures depends on the catabolite repression properties of Saccharomyces cerevisiae

AU - Cortassa, Sonia

AU - Aon, Miguel A.

PY - 1998/6

Y1 - 1998/6

N2 - In glucose-limited continuous cultures, a Crabtree positive yeast such as Saccharomyces cerevisiae displays respiratory metabolism at low dilution rates (D) and respirofermentative metabolism at high D. We hypothesized that the onset of fermentative metabolism is related with the catabolite repression or glucose repression effect. To test this hypothesis, we have investigated the physiological behaviour in glucose-limited continuous cultures of S.cerevisiae strain CEN.PK122 and isogenic mutants, snf1 (cat1) and snf4 (cat3), defective in proteins involved in the release from glucose repression and the mutant in glucose repression mig1. We analyzed the behaviour of the wild type and mutant strains at steady state in chemostat cultures as a function of D. Wild-type cells displayed respiratory metabolism up to a D of 0.2 h-1. snf1 and snf4 mutants started fermenting after a D of 0.1 and 0.15 h-1, respectively. The latter behavior was not due to an impairment of respiration since their specific rate of oxygen consumption was similar or even higher than that shown by the wild type and the other mutants in the whole range of D studied. We conclude that the onset of fermentative metabolism in yeast growing in chemostat cultures is related with glucose repression.

AB - In glucose-limited continuous cultures, a Crabtree positive yeast such as Saccharomyces cerevisiae displays respiratory metabolism at low dilution rates (D) and respirofermentative metabolism at high D. We hypothesized that the onset of fermentative metabolism is related with the catabolite repression or glucose repression effect. To test this hypothesis, we have investigated the physiological behaviour in glucose-limited continuous cultures of S.cerevisiae strain CEN.PK122 and isogenic mutants, snf1 (cat1) and snf4 (cat3), defective in proteins involved in the release from glucose repression and the mutant in glucose repression mig1. We analyzed the behaviour of the wild type and mutant strains at steady state in chemostat cultures as a function of D. Wild-type cells displayed respiratory metabolism up to a D of 0.2 h-1. snf1 and snf4 mutants started fermenting after a D of 0.1 and 0.15 h-1, respectively. The latter behavior was not due to an impairment of respiration since their specific rate of oxygen consumption was similar or even higher than that shown by the wild type and the other mutants in the whole range of D studied. We conclude that the onset of fermentative metabolism in yeast growing in chemostat cultures is related with glucose repression.

KW - Catabolite repression mutants

KW - Chemostat cultures

KW - Fermentation

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=0032103149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032103149&partnerID=8YFLogxK

U2 - 10.1016/S0141-0229(97)00268-8

DO - 10.1016/S0141-0229(97)00268-8

M3 - Article

AN - SCOPUS:0032103149

VL - 22

SP - 705

EP - 712

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 8

ER -