The O139 serogroup of Vibrio cholerae comprises diverse clones of epidemic and nonepidemic strains derived from multiple V. cholerae O1 or non-O1 progenitors

S. M. Faruque, M. N. Saha, Asadulghani, D. A. Sack, R. B. Sack, Y. Takeda, G. B. Nair

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Abstract

Sixty-four representative strains of Vibrio cholerae O139 were analyzed, to re-examine the origin of this serogroup. Ribotyping differentiated the strains into 3 HindIII and 7 BglI ribotypes. One HindIII and 5 BglI ribotypes were shared by all toxigenic O139 strains. Of 6 nontoxigenic O139 strains, 3 shared ribotypes with the toxigenic strains, carried genes encoding toxin coregulated pilus, and were susceptible to the cholera toxin-converting bacteriophage CTXΦ. The remaining 3 strains belonged to 2 different ribotypes distinct from toxigenic O139 strains and were resistant to CTXΦ, and JA-1, an O139-specific lyric bacteriophage. Polymerase chain reaction amplicons corresponding to the gmhD gene carried by these 3 strains also differed from those of the toxigenic O139 strains but were identical to those of 15 environmental non-O1-non-O139 strains. Thus, the O139 antigen is present in different lineages, and this serogroup appears to comprise epidemic and nonepidemic strains derived separately from different progenitors.

Original languageEnglish (US)
Pages (from-to)1161-1168
Number of pages8
JournalJournal of Infectious Diseases
Volume182
Issue number4
DOIs
Publication statusPublished - 2000

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ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

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