The nucleotide sequence of the gB glycoprotein gene of HSV-2 and comparison with the corresponding gene of HSV-1

David J. Bzik, Chitrita Debroy, Barbara A. Fox, Nels E. Pederson, Stanley Person

Research output: Contribution to journalArticle

Abstract

The nucleotide sequence of the gB glycoprotein gene of HSV-2 has been determined and compared with the homologous gene of HSV-1. The two genes are specified by the same total number of codons (904); eight additional codons of the HSV-1 gene are found within the signal sequence, and eight additional codons of the HSV-2 gene are found at three different sites in the gene. The signal cleavage, membrane-spanning, and eight potential N-linked oligosaccharide sites, as well as 5′- and 3′-regulatory signals are largely conserved. The overall amino acid homology is 85%;least conserved are the N- and C-terminal regions of the protein. Secondary structure plots were determined for the two proteins, and the structures were compared with each other and with alterations in structure due to several mutations in the HSV-1 gB gene for which sequence analysis is available. The high homology in primary and secondary structure suggests a conserved, essential function for the gene.

Original languageEnglish (US)
Pages (from-to)322-333
Number of pages12
JournalVirology
Volume155
Issue number2
DOIs
StatePublished - 1986
Externally publishedYes

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Human Herpesvirus 2
Human Herpesvirus 1
Glycoproteins
Genes
Codon
Essential Genes
Protein Sorting Signals
Oligosaccharides
Sequence Analysis
Proteins
Amino Acids
Mutation
Membranes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

The nucleotide sequence of the gB glycoprotein gene of HSV-2 and comparison with the corresponding gene of HSV-1. / Bzik, David J.; Debroy, Chitrita; Fox, Barbara A.; Pederson, Nels E.; Person, Stanley.

In: Virology, Vol. 155, No. 2, 1986, p. 322-333.

Research output: Contribution to journalArticle

Bzik, David J. ; Debroy, Chitrita ; Fox, Barbara A. ; Pederson, Nels E. ; Person, Stanley. / The nucleotide sequence of the gB glycoprotein gene of HSV-2 and comparison with the corresponding gene of HSV-1. In: Virology. 1986 ; Vol. 155, No. 2. pp. 322-333.
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