The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy

George H. Sack; Opendra Narayan; Kathleen J. Danna; Leslie P. Weiner; Daniel Nathans

doi:10.1016/0042-6822(73)90433-9

The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy

George H. Sack, Opendra Narayan, Kathleen J. Danna, Leslie P. Weiner, Daniel Nathans

School of Medicine

Research output: Contribution to journal › Article › peer-review

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Abstract

The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy has been compared with the DNA of a laboratory strain of SV40. DNA of this isolate (designated PML-2) is a covalently closed circular duplex with the same sedimentation rate as SV40 DNA. Denatured PML-2 DNA hybridizes with denatured SV40 DNA at about the same rate as homologous DNA. When digested with Hemophilus influenzae restriction endonuclease, PML-2 DNA yielded 11 fragments separable by electrophoresis, nine of which co-migrated with fragments of SV40 DNA, and two of which did not. We conclude that the PML-2 virus is a variant of SV40.

Original language	English (US)
Pages (from-to)	345-350
Number of pages	6
Journal	Virology
Volume	51
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(73)90433-9
State	Published - Feb 1973

ASJC Scopus subject areas

Virology

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title = "The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy",

abstract = "The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy has been compared with the DNA of a laboratory strain of SV40. DNA of this isolate (designated PML-2) is a covalently closed circular duplex with the same sedimentation rate as SV40 DNA. Denatured PML-2 DNA hybridizes with denatured SV40 DNA at about the same rate as homologous DNA. When digested with Hemophilus influenzae restriction endonuclease, PML-2 DNA yielded 11 fragments separable by electrophoresis, nine of which co-migrated with fragments of SV40 DNA, and two of which did not. We conclude that the PML-2 virus is a variant of SV40.",

author = "Sack, {George H.} and Opendra Narayan and Danna, {Kathleen J.} and Weiner, {Leslie P.} and Daniel Nathans",

note = "Funding Information: This research has been supported by grants from the National Institutes of Health, U.S. Public Health Service (CA11895 and NS08838) and from the Whitehall Foundation, Inc. G.H.S. is supported by U.S.P.H.S. Training Grant GMO0624; O.N., by a gift from the Hamilton Roddis Foundation; and L.P.W., by a Career Development Award (NS50274) from the U.S.P.H.S.",

year = "1973",

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doi = "10.1016/0042-6822(73)90433-9",

language = "English (US)",

volume = "51",

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journal = "Virology",

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T1 - The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy

AU - Sack, George H.

AU - Narayan, Opendra

AU - Danna, Kathleen J.

AU - Weiner, Leslie P.

AU - Nathans, Daniel

N1 - Funding Information: This research has been supported by grants from the National Institutes of Health, U.S. Public Health Service (CA11895 and NS08838) and from the Whitehall Foundation, Inc. G.H.S. is supported by U.S.P.H.S. Training Grant GMO0624; O.N., by a gift from the Hamilton Roddis Foundation; and L.P.W., by a Career Development Award (NS50274) from the U.S.P.H.S.

PY - 1973/2

Y1 - 1973/2

N2 - The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy has been compared with the DNA of a laboratory strain of SV40. DNA of this isolate (designated PML-2) is a covalently closed circular duplex with the same sedimentation rate as SV40 DNA. Denatured PML-2 DNA hybridizes with denatured SV40 DNA at about the same rate as homologous DNA. When digested with Hemophilus influenzae restriction endonuclease, PML-2 DNA yielded 11 fragments separable by electrophoresis, nine of which co-migrated with fragments of SV40 DNA, and two of which did not. We conclude that the PML-2 virus is a variant of SV40.

AB - The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy has been compared with the DNA of a laboratory strain of SV40. DNA of this isolate (designated PML-2) is a covalently closed circular duplex with the same sedimentation rate as SV40 DNA. Denatured PML-2 DNA hybridizes with denatured SV40 DNA at about the same rate as homologous DNA. When digested with Hemophilus influenzae restriction endonuclease, PML-2 DNA yielded 11 fragments separable by electrophoresis, nine of which co-migrated with fragments of SV40 DNA, and two of which did not. We conclude that the PML-2 virus is a variant of SV40.

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The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy

Abstract

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