The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy has been compared with the DNA of a laboratory strain of SV40. DNA of this isolate (designated PML-2) is a covalently closed circular duplex with the same sedimentation rate as SV40 DNA. Denatured PML-2 DNA hybridizes with denatured SV40 DNA at about the same rate as homologous DNA. When digested with Hemophilus influenzae restriction endonuclease, PML-2 DNA yielded 11 fragments separable by electrophoresis, nine of which co-migrated with fragments of SV40 DNA, and two of which did not. We conclude that the PML-2 virus is a variant of SV40.
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