TY - JOUR
T1 - The nuclear receptor seven up regulates genes involved in immunity and xenobiotic response in the adult drosophila female fat body
AU - Weaver, Lesley N.
AU - Drummond-Barbosa, Daniela
N1 - Funding Information:
Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40 OD018537) and the Vienna Drosophila Resource Center (VDRC, www.vdrc.at) (Dietzl et al. 2007) were used in this study. We are thankful to Flybase (www.flybase.org), an essential Drosophila research resource. We are grateful to members of the Drummond-Barbosa Lab for critical reading of the manuscript. This work was supported by National Institutes of Health R01 GM069875 (D.D.-B.) and Pathway to Independence Award K99 GM127605 and R00 GM127605 (L.N.W.) grants.
Publisher Copyright:
Copyright © 2020 Weaver, Drummond-Barbosa
PY - 2020/12
Y1 - 2020/12
N2 - The physiology of organisms depends on inter-organ communication in response to changes in the environment. Nuclear receptors are broadly expressed transcription factors that respond to circulating molecules to control many biological processes, including immunity, detoxification, and reproduction. Although the tissue-intrinsic roles of nuclear receptors in reproduction have been extensively studied, there is increasing evidence that nuclear receptor signaling in peripheral tissues can also influence oogenesis. We previously showed that the Drosophila nuclear receptor Seven up (Svp) is required in the adult fat body to regulate distinct steps of oogenesis; however, the relevant downstream targets of Svp remain unknown. Here, we took an RNA sequencing approach to identify candidate Svp targets specifically in the adult female fat body that might mediate this response. svp knockdown in the adult female fat body significantly downregulated immune genes involved in the first line of pathogen defense, suggesting a role for Svp in stimulating early immunity. In addition, we found that Svp transcriptionally regulates genes involved in each step of the xenobiotic detoxification response. Based on these findings, we propose a testable model in which Svp functions in the adult female fat body to stimulate early defense against pathogens and facilitate detoxification as part of its mechanisms to promote oogenesis.
AB - The physiology of organisms depends on inter-organ communication in response to changes in the environment. Nuclear receptors are broadly expressed transcription factors that respond to circulating molecules to control many biological processes, including immunity, detoxification, and reproduction. Although the tissue-intrinsic roles of nuclear receptors in reproduction have been extensively studied, there is increasing evidence that nuclear receptor signaling in peripheral tissues can also influence oogenesis. We previously showed that the Drosophila nuclear receptor Seven up (Svp) is required in the adult fat body to regulate distinct steps of oogenesis; however, the relevant downstream targets of Svp remain unknown. Here, we took an RNA sequencing approach to identify candidate Svp targets specifically in the adult female fat body that might mediate this response. svp knockdown in the adult female fat body significantly downregulated immune genes involved in the first line of pathogen defense, suggesting a role for Svp in stimulating early immunity. In addition, we found that Svp transcriptionally regulates genes involved in each step of the xenobiotic detoxification response. Based on these findings, we propose a testable model in which Svp functions in the adult female fat body to stimulate early defense against pathogens and facilitate detoxification as part of its mechanisms to promote oogenesis.
KW - Drosophila
KW - Fat body
KW - Immunity
KW - Oogenesis
KW - Seven up
KW - Xenobiotics
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U2 - 10.1534/g3.120.401745
DO - 10.1534/g3.120.401745
M3 - Article
C2 - 33087412
AN - SCOPUS:85097211458
SN - 2160-1836
VL - 10
SP - 4625
EP - 4635
JO - G3: Genes, Genomes, Genetics
JF - G3: Genes, Genomes, Genetics
IS - 12
ER -