TY - JOUR
T1 - The na+/h+ exchanger role in the intracellular ph regulation of human platelets
AU - Gende, O. A.
AU - Cingolani, H. E.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1991
Y1 - 1991
N2 - The intracellular pH of human platelets was measured with a fluorescent intracellular probe. When platelets were in basal conditions (pHo 7.4, [Na+]o 140 mm) the pHi was 6.98 ± 0.04. Five minutes after the addition of EIPA 60 μm, an inhibitor of Na+/H+ exchange, the pHi fell in 0.075 ± 0.022 pH units (P < 0.05). Preincubation in a sodium free, acid medium (pHo 6.3) induced a cell acidification to pH 6.61 ± 0.03 (P < 0.01). Preacidified platelets showed a recovery in sodium-containing solution that is a function of [Na+]o. The initial rate of recovery depends on [Na+]o in a Michaelis-Menten fashion, showing a Km of 35.6 mm and a Vmax of 0.213 pH units/min. These results show that the pHi is maintained in human platelets by a Na+/H+exchange that is active even in basal conditions. The properties of the Na+/H+ exchanger in human platelets and in the less accessible smooth vascular cells are similar; generalized pathological alterations, like hypertension, could be reflected by both tissues.
AB - The intracellular pH of human platelets was measured with a fluorescent intracellular probe. When platelets were in basal conditions (pHo 7.4, [Na+]o 140 mm) the pHi was 6.98 ± 0.04. Five minutes after the addition of EIPA 60 μm, an inhibitor of Na+/H+ exchange, the pHi fell in 0.075 ± 0.022 pH units (P < 0.05). Preincubation in a sodium free, acid medium (pHo 6.3) induced a cell acidification to pH 6.61 ± 0.03 (P < 0.01). Preacidified platelets showed a recovery in sodium-containing solution that is a function of [Na+]o. The initial rate of recovery depends on [Na+]o in a Michaelis-Menten fashion, showing a Km of 35.6 mm and a Vmax of 0.213 pH units/min. These results show that the pHi is maintained in human platelets by a Na+/H+exchange that is active even in basal conditions. The properties of the Na+/H+ exchanger in human platelets and in the less accessible smooth vascular cells are similar; generalized pathological alterations, like hypertension, could be reflected by both tissues.
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U2 - 10.3109/13813459109145910
DO - 10.3109/13813459109145910
M3 - Article
C2 - 1713493
AN - SCOPUS:0026113598
SN - 1381-3455
VL - 99
SP - 95
EP - 98
JO - Archives of Physiology and Biochemistry
JF - Archives of Physiology and Biochemistry
IS - 1
ER -